Proliferating cell nuclear antigen (PCNA) allows the automatic identification of follicles in microscopic images of human ovarian tissue
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Background: Human ovarian reserve is defined by the population of nongrowing follicles (NGFs) in the ovary. Direct estimation of ovarian reserve involves the identification of NGFs in prepared ovarian tissue. Previous studies involving human tissue have used hematoxylin and eosin (HE) stain, with NGF populations estimated by human examination either of tissue under a microscope, or of images taken of this tissue. Methods: In this study we replaced HE with proliferating cell nuclear antigen (PCNA), and automated the identification and enumeration of NGFs that appear in the resulting microscopic images. We compared the automated estimates to those obtained by human experts, with the “gold standard” taken to be the average of the conservative and liberal estimates by three human experts. Results: The automated estimates were within 10% of the “gold standard”, for images at both 100× and 200× magnifications. Automated analysis took longer than human analysis for several hundred images, not allowing for breaks from analysis needed by humans. Conclusion: Our results both replicate and improve on those of previous studies involving rodent ovaries, and demonstrate the viability of large-scale studies of human ovarian reserve using a combination of immunohistochemistry and computational image analysis techniques.
Kelsey , T W , Caserta , B , Castillo , L , Wallace , W H B & Coppola , F 2010 , ' Proliferating cell nuclear antigen (PCNA) allows the automatic identification of follicles in microscopic images of human ovarian tissue ' Journal of Pathology and Laboratory Medicine International , vol. 2010 , no. 2 , pp. 99-105 . DOI: 10.2147/PLMI.S11116
Journal of Pathology and Laboratory Medicine International
© 2010 Kelsey et al, publisher and licensee Dove Medical Press Ltd. This is an Open Access article which permits unrestricted noncommercial use, provided the original work is properly cited. This work is published by Dove Medical Press Limited, and licensed under Creative Commons Attribution - Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/).
DescriptionTWK is supported by EPSRC grants EP/CS23229/1 and EP/H004092/1.
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