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dc.contributor.authorWhite, Malcolm Frederick
dc.contributor.authorLilley, DMJ
dc.date.accessioned2014-07-04T16:01:01Z
dc.date.available2014-07-04T16:01:01Z
dc.date.issued1997-11
dc.identifier.citationWhite , M F & Lilley , DMJ 1997 , ' Characterisation of a Holliday junction-resolving enzyme from Schizosaccharomyces pombe ' Molecular and Cellular Biology , vol. 17 , no. 11 , pp. 6465-6471 .en
dc.identifier.issn0270-7306
dc.identifier.otherPURE: 98563
dc.identifier.otherPURE UUID: 51a23fd9-2c3d-4b85-9414-7ed40f9a98ab
dc.identifier.otherWOS: A1997YC25500029
dc.identifier.otherScopus: 0030857980
dc.identifier.otherORCID: /0000-0003-1543-9342/work/47136081
dc.identifier.urihttp://hdl.handle.net/10023/4953
dc.identifier.urihttp://www.scopus.com/inward/record.url?scp=0030857980&partnerID=8YFLogxKen
dc.identifier.urihttp://europepmc.org/abstract/MED/9343409en
dc.identifier.urihttp://mcb.asm.org/content/17/11/6465.abstract?sid=f572b001-2195-4c45-a774-236a1b4ba119en
dc.descriptionWe thank the Cancer Research Campaign for financial support.en
dc.description.abstractThe rearrangement and repair of DNA by homologous recombination involves the creation of Holliday junctions, which are cleaved by a class of junction-specific endonucleases to generate recombinant duplex DNA products, Only two cellular junction-resolving enzymes have been identified to date: RuvC in eubacteria and CCE1 from Saccharomyces cerevisiae mitochondria, We have identified a protein from Schizosaccharomyces pombe which has 28% sequence identity to CCE1. The YDC2 protein has been, cloned and overexpressed in Escherichia coli, and the purified recombinant protein has been shown to be a Holliday junction-resolving enzyme. YDC2 has a high degree of specificity for the structure of the four-way junction, to which it binds as a dimer, The enzyme exhibits a sequence specificity for junction cleavage that differs from both CCE1 and RuvC, and it cleaves fixed junctions at the point of strand exchange, The conservation of the mechanism of Holliday junction cleavage between two organisms as diverse as S. cerevisiae and S. pombe suggests that there may be a common pathway for mitochondrial homologous recombination in fungi, plants, protists, and possibly higher eukaryotes.en
dc.format.extent7en
dc.language.isoeng
dc.relation.ispartofMolecular and Cellular Biologyen
dc.rightsCopyright © 1997, American Society for Microbiology. Open Access article available on Europe PMC.en
dc.subjectCruciform cutting endonucleaseen
dc.subjectSaccharomyces-cerevisiaeen
dc.subjectEscherichia-colien
dc.subjectMitochondrial-dnaen
dc.subjectIn-vitroen
dc.subjectGenetic-recombinationen
dc.subjectRuvc resolvaseen
dc.subjectResolutionen
dc.subjectProteinen
dc.subjectYeasten
dc.subjectQR Microbiologyen
dc.subject.lccQRen
dc.titleCharacterisation of a Holliday junction-resolving enzyme from Schizosaccharomyces pombeen
dc.typeJournal articleen
dc.description.versionPublisher PDFen
dc.contributor.institutionUniversity of St Andrews. School of Biologyen
dc.contributor.institutionUniversity of St Andrews. Biomedical Sciences Research Complexen
dc.description.statusPeer revieweden


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