Files in this item
Characterisation of a Holliday junction-resolving enzyme from Schizosaccharomyces pombe
Item metadata
dc.contributor.author | White, Malcolm Frederick | |
dc.contributor.author | Lilley, DMJ | |
dc.date.accessioned | 2014-07-04T16:01:01Z | |
dc.date.available | 2014-07-04T16:01:01Z | |
dc.date.issued | 1997-11 | |
dc.identifier.citation | White , M F & Lilley , DMJ 1997 , ' Characterisation of a Holliday junction-resolving enzyme from Schizosaccharomyces pombe ' , Molecular and Cellular Biology , vol. 17 , no. 11 , pp. 6465-6471 . | en |
dc.identifier.issn | 0270-7306 | |
dc.identifier.other | PURE: 98563 | |
dc.identifier.other | PURE UUID: 51a23fd9-2c3d-4b85-9414-7ed40f9a98ab | |
dc.identifier.other | WOS: A1997YC25500029 | |
dc.identifier.other | Scopus: 0030857980 | |
dc.identifier.other | ORCID: /0000-0003-1543-9342/work/47136081 | |
dc.identifier.uri | https://hdl.handle.net/10023/4953 | |
dc.description | We thank the Cancer Research Campaign for financial support. | en |
dc.description.abstract | The rearrangement and repair of DNA by homologous recombination involves the creation of Holliday junctions, which are cleaved by a class of junction-specific endonucleases to generate recombinant duplex DNA products, Only two cellular junction-resolving enzymes have been identified to date: RuvC in eubacteria and CCE1 from Saccharomyces cerevisiae mitochondria, We have identified a protein from Schizosaccharomyces pombe which has 28% sequence identity to CCE1. The YDC2 protein has been, cloned and overexpressed in Escherichia coli, and the purified recombinant protein has been shown to be a Holliday junction-resolving enzyme. YDC2 has a high degree of specificity for the structure of the four-way junction, to which it binds as a dimer, The enzyme exhibits a sequence specificity for junction cleavage that differs from both CCE1 and RuvC, and it cleaves fixed junctions at the point of strand exchange, The conservation of the mechanism of Holliday junction cleavage between two organisms as diverse as S. cerevisiae and S. pombe suggests that there may be a common pathway for mitochondrial homologous recombination in fungi, plants, protists, and possibly higher eukaryotes. | |
dc.format.extent | 7 | |
dc.language.iso | eng | |
dc.relation.ispartof | Molecular and Cellular Biology | en |
dc.rights | Copyright © 1997, American Society for Microbiology. Open Access article available on Europe PMC. | en |
dc.subject | Cruciform cutting endonuclease | en |
dc.subject | Saccharomyces-cerevisiae | en |
dc.subject | Escherichia-coli | en |
dc.subject | Mitochondrial-dna | en |
dc.subject | In-vitro | en |
dc.subject | Genetic-recombination | en |
dc.subject | Ruvc resolvase | en |
dc.subject | Resolution | en |
dc.subject | Protein | en |
dc.subject | Yeast | en |
dc.subject | QR Microbiology | en |
dc.subject.lcc | QR | en |
dc.title | Characterisation of a Holliday junction-resolving enzyme from Schizosaccharomyces pombe | en |
dc.type | Journal article | en |
dc.description.version | Publisher PDF | en |
dc.contributor.institution | University of St Andrews. School of Biology | en |
dc.contributor.institution | University of St Andrews. Biomedical Sciences Research Complex | en |
dc.description.status | Peer reviewed | en |
dc.identifier.url | http://www.scopus.com/inward/record.url?scp=0030857980&partnerID=8YFLogxK | en |
dc.identifier.url | http://europepmc.org/abstract/MED/9343409 | en |
dc.identifier.url | http://mcb.asm.org/content/17/11/6465.abstract?sid=f572b001-2195-4c45-a774-236a1b4ba119 | en |
This item appears in the following Collection(s)
Items in the St Andrews Research Repository are protected by copyright, with all rights reserved, unless otherwise indicated.