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Common properties of the nuclear body protein SP100 and the TIF1alpha chromatin factor: the role of SUMO modification

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Hay_2001_MCB_Common.pdf (843.3Kb)
Date
05/2001
Author
Seeler, Jacob-S
Marchio, Agnes
Losson, Regine
Desterro, Joana M P
Hay, Ronald Thomas
Chambon, Pierre
Dejean, Anne
Keywords
Acute promyelocytic leukemia
Primary biliary-cirrhosis
Zinc-finger proteins
Pml-rar-alpha
Covalent modification
Transcriptional coactivator
Repressor domain
Binding-protein
Pore complex
HP1 proteins
QR Microbiology
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Abstract
The SP100 protein, together with PML, represents a major constituent of the PML-SP100 nuclear bodies (NBs). The function of these ubiquitous subnuclear structures, whose integrity is compromised in pathological situations such as acute promyelocytic leukemia (APL) or DNA virus infection, remains poorly understood. There is little evidence for the occurrence of actual physiological processes within NBs. The two NE proteins PML and SP100 are covalently modified by the ubiquitin-related SUMO-1 modifier, and recent work indicates that this modification is critical for the regulation of NE dynamics. In exploring the functional relationships between NBs and chromatin, we have shown previously that SP100 interacts with members of the HP1 family of nonhistone chromosomal proteins and that a variant SP100 cDNA encodes a high-mobility group (HMG1/2) protein. Here we report the isolation of a further cDNA, encoding the SP100C protein, that contains the PHD-bromodomain motif characteristic of chromatin proteins. We further show that TIF1 alpha, a chromatin-associated factor with homology to both PML and SP100C, is also modified by SUMO-1. Finally, in vitro experiments indicate that SUMO modification of SP100 enhances the stability of SP100-HP1 complexes. Taken together, our results suggest an association of SP100 and its variants with the chromatin compartment and, further, indicate that SUMO modification may play a regulatory role in the functional interplay between the nuclear bodies and chromatin.
Citation
Seeler , J-S , Marchio , A , Losson , R , Desterro , J M P , Hay , R T , Chambon , P & Dejean , A 2001 , ' Common properties of the nuclear body protein SP100 and the TIF1alpha chromatin factor: the role of SUMO modification ' , Molecular and Cellular Biology , vol. 21 , no. 10 , pp. 3314-3324 . https://doi.org/10.1128/MCB.21.10.3314-3324.2001
Publication
Molecular and Cellular Biology
Status
Peer reviewed
DOI
https://doi.org/10.1128/MCB.21.10.3314-3324.2001
ISSN
0270-7306
Type
Journal article
Rights
Copyright © 2001, American Society for Microbiology. All Rights Reserved. Open Access article available from PMC.
Description
This work was supported by grants from the European Community, the Association for International Cancer Research, and the Association pour la Recherche sur le Cancer.
Collections
  • University of St Andrews Research
URL
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC100253/
URI
http://hdl.handle.net/10023/4944

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