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p68 RNA helicase - identification of a nucleolar form and cloning of related genes containing a conserved intron in yeasts

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Macneill_1990_MCB_p68.pdf (2.283Mb)
Date
03/1991
Author
Iggo, RD
Jamieson, DJ
MacNeill, Stuart Andrew
Southgate, J
McPheat, J
Lane, DP
Keywords
A-D box
Schizosaccharomyces-pombe
Saccharomyces-cerevisiae
Protein
Sequence
Nuclear
DNA
localisation
Transformation
Replication
QR Microbiology
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Abstract
The human p68 protein is an RNA-dependent ATPase and RNA helicase which was first identified because of its immunological cross-reaction with a viral RNA helicase, simian virus 40 large T antigen. It belongs to a recently discovered family of proteins (DEAD box proteins) that share extensive regions of amino acid sequence homology, are ubiquitous in living organisms, and are involved in many aspects of RNA metabolism, including splicing, translation, and ribosome assembly. We have shown by immunofluorescent microscopy that mammalian p68, which is excluded from the nucleoli during interphase, translocates to prenucleolar bodies during telophase. We have cloned 55% identical genes from both Schizosaccharomyces pombe and Saccharomyces cerevisiae and shown that they are essential in both yeasts. The human and yeast genes contain a large intron whose position has been precisely conserved. In S. cerevisiae, the intron is unusual both because of its size and because of its location near the 3' end of the gene. We discuss possible functional roles for such an unusual intron in an RNA helicase gene.
Citation
Iggo , RD , Jamieson , DJ , MacNeill , S A , Southgate , J , McPheat , J & Lane , DP 1991 , ' p68 RNA helicase - identification of a nucleolar form and cloning of related genes containing a conserved intron in yeasts ' , Molecular and Cellular Biology , vol. 11 , no. 3 , pp. 1326-1333 .
Publication
Molecular and Cellular Biology
Status
Peer reviewed
ISSN
0270-7306
Type
Journal article
Rights
Copyright © 1991, American Society for Microbiology. Open Access article available on PMC.
Collections
  • University of St Andrews Research
URL
http://www.scopus.com/inward/record.url?scp=0025978633&partnerID=8YFLogxK
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC369403/
URI
http://hdl.handle.net/10023/4940

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