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dc.contributor.authorRima, Bert K
dc.contributor.authorGatherer, Derek
dc.contributor.authorYoung, Daniel F
dc.contributor.authorNorsted, Hanna
dc.contributor.authorRandall, Richard E
dc.contributor.authorDavison, Andrew J
dc.identifier.citationRima , B K , Gatherer , D , Young , D F , Norsted , H , Randall , R E & Davison , A J 2014 , ' Stability of the parainfluenza virus 5 genome revealed by deep sequencing of strains isolated from different hosts and following passage in cell culture ' , Journal of Virology , vol. 88 , no. 7 , pp. 3826-3836 .
dc.identifier.otherPURE: 127302923
dc.identifier.otherPURE UUID: da4a215f-c359-49ec-b150-638ab45bd6ec
dc.identifier.otherPubMed: 24453358
dc.identifier.otherScopus: 84895456203
dc.identifier.otherORCID: /0000-0002-9304-6678/work/60427040
dc.identifier.otherWOS: 000332624700018
dc.descriptionThis work was supported by the Wellcome Trust (D.F.Y. and R.E.R.), the United Kingdom Medical Research Council (A.J.D. and D.G.), and the University of St. Andrews (H.N.).en
dc.description.abstractThe strain diversity of a rubulavirus, parainfluenza virus 5 (PIV5), was investigated by comparing 11 newly determined and 6 previously published genome sequences. These sequences represent 15 PIV5 strains, of which 6 were isolated from humans, 1 was from monkeys, 2 were from pigs, and 6 were from dogs. Strain diversity is remarkably low, regardless of host, year of isolation, or geographical origin; a total of 7.8% of nucleotides are variable, and the average pairwise difference between strains is 2.1%. Variation is distributed unevenly across the PIV5 genome, but no convincing evidence of selection for antibody-mediated evasion in hemagglutinin-neuraminidase was found. The finding that some canine and porcine, but not primate, strains are mutated in the SH gene, and do not produce SH, raised the possibility that dogs (or pigs) may not be the natural host of PIV5. The genetic stability of PIV5 was also demonstrated during serial passage of one strain (W3) in Vero cells at a high multiplicity of infection, under conditions of competition with large proportions of defective interfering genomes. A similar observation was made for a strain W3 mutant (PIV5VΔC) lacking V gene function, in which the dominant changes were related to pseudoreversion in this gene. The mutations detected in PIV5VΔC during pseudoreversion, and also those characterizing the SH gene in canine and porcine strains, predominantly involved U-to-C transitions. This suggests an important role for biased hypermutation via an adenosine deaminase, RNA-specific (ADAR)-like activity.
dc.relation.ispartofJournal of Virologyen
dc.rightsCopyright © 2014 Rima et al. This is an open-access article distributed under the terms of the Creative Commons Attribution 3.0 Unported license.
dc.subjectGenetic Variationen
dc.subjectGenomic Instabilityen
dc.subjectHigh-Throughput Nucleotide Sequencingen
dc.subjectMolecular Sequence Dataen
dc.subjectParainfluenza Virus 5en
dc.subjectRubulavirus Infectionsen
dc.subjectSerial Passageen
dc.subjectVirus Cultivationen
dc.subjectQR355 Virologyen
dc.titleStability of the parainfluenza virus 5 genome revealed by deep sequencing of strains isolated from different hosts and following passage in cell cultureen
dc.typeJournal articleen
dc.description.versionPublisher PDFen
dc.contributor.institutionUniversity of St Andrews. School of Biologyen
dc.contributor.institutionUniversity of St Andrews. Biomedical Sciences Research Complexen
dc.description.statusPeer revieweden

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