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Wzi Is an Outer Membrane Lectin that Underpins Group 1 Capsule Assembly in Escherichia coli

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Date
25/04/2013
Author
Bushell, Simon
Mainprize, Iain L.
Wear, Martin A.
Lou, Hubing
Whitfield, Chris
Naismith, Jim
Funder
The Wellcome Trust
Grant ID
081862/Z/06/Z
Keywords
Surface-plasmon resonance
Colanic acid biosynthesis
Gram-negative bacteria
Klebsiella-pneumoniae
K antigens
Functional-analysis
Crystal-structure
Structural basis
Gene-expression
Concanavalin-A
QH301 Biology
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Abstract
Many pathogenic bacteria encase themselves in a polysaccharide capsule that provides a barrier to the physical and immunological challenges of the host. The mechanism by which the capsule assembles around the bacterial cell is unknown. Wzi, an integral outer-membrane protein from Escherichia coli, has been implicated in the formation of group 1 capsules. The 2.6 angstrom resolution structure of Wzi reveals an 18-stranded beta-barrel fold with a novel arrangement of long extracellular loops that blocks the extracellular entrance and a helical bundle that plugs the periplasmic end. Mutagenesis shows that specific extracellular loops are required for in vivo capsule assembly. The data show that Wzi binds the K30 carbohydrate polymer and, crucially, that mutants functionally deficient in vivo show no binding to K30 polymer in vitro. We conclude that Wzi is a novel outer-membrane lectin that assists in the formation of the bacterial capsule via direct interaction with capsular polysaccharides.
Citation
Bushell , S , Mainprize , I L , Wear , M A , Lou , H , Whitfield , C & Naismith , J 2013 , ' Wzi Is an Outer Membrane Lectin that Underpins Group 1 Capsule Assembly in Escherichia coli ' , Structure , vol. 21 , no. 5 , pp. 844-853 . https://doi.org/10.1016/j.str.2013.03.010
Publication
Structure
Status
Peer reviewed
DOI
https://doi.org/10.1016/j.str.2013.03.010
ISSN
0969-2126
Type
Journal article
Rights
© The Authors. This is an open access article, available from http://www.sciencedirect.com
Collections
  • University of St Andrews Research
URL
http://europepmc.org/articles/PMC3791409
URI
http://hdl.handle.net/10023/4797

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