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Nitrosopersulfide (SSNO) accounts for sustained NO bioactivity of S-nitrosothiols following reaction with sulfide
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dc.contributor.author | Cortese-Krott, M.M. | |
dc.contributor.author | Fernandez, B.O. | |
dc.contributor.author | Santos, J.L.T. | |
dc.contributor.author | Mergia, E. | |
dc.contributor.author | Grman, M. | |
dc.contributor.author | Nagy, P. | |
dc.contributor.author | Kelm, M. | |
dc.contributor.author | Butler, A. | |
dc.contributor.author | Feelisch, M. | |
dc.date.accessioned | 2014-05-13T11:01:04Z | |
dc.date.available | 2014-05-13T11:01:04Z | |
dc.date.issued | 2014-01 | |
dc.identifier | 118128828 | |
dc.identifier | a7238199-87e9-4080-a838-f7c279f8fd22 | |
dc.identifier | 84893371806 | |
dc.identifier | 000350769600030 | |
dc.identifier.citation | Cortese-Krott , M M , Fernandez , B O , Santos , J L T , Mergia , E , Grman , M , Nagy , P , Kelm , M , Butler , A & Feelisch , M 2014 , ' Nitrosopersulfide (SSNO) accounts for sustained NO bioactivity of S-nitrosothiols following reaction with sulfide ' , Redox Biology , vol. 2 , no. 1 , pp. 234-244 . https://doi.org/10.1016/j.redox.2013.12.031 | en |
dc.identifier.issn | 2213-2317 | |
dc.identifier.uri | https://hdl.handle.net/10023/4772 | |
dc.description | Funding: Susanne-Bunnenberg-Stiftung of the Düsseldorf Heart Center (to MK), the COSTaction BM1005 (European Network on Gasotransmitters), the FP7 Marie Curie International Reintegration program (PIRG08-GA-2010-277006, to PN) and the Faculty of Medicine, University of Southampton (to MF). | en |
dc.description.abstract | Sulfide salts are known to promote the release of nitric oxide (NO) from S-nitrosothiols and potentiate their vasorelaxant activity, but much of the cross-talk between hydrogen sulfide and NO is believed to occur via functional interactions of cell regulatory elements such as phosphodiesterases. Using RFL-6 cells as an NO reporter system we sought to investigate whether sulfide can also modulate nitrosothiol-mediated soluble guanylyl cyclase (sGC) activation following direct chemical interaction. We find a U-shaped dose response relationship where low sulfide concentrations attenuate sGC stimulation by S-nitrosopenicillamine (SNAP) and cyclic GMP levels are restored at equimolar ratios. Similar results are observed when intracellular sulfide levels are raised by pre-incubation with the sulfide donor, GYY4137. The outcome of direct sulfide/nitrosothiol interactions also critically depends on molar reactant ratios and is accompanied by oxygen consumption. With sulfide in excess, a 'yellow compound' accumulates that is indistinguishable from the product of solid-phase transnitrosation of either hydrosulfide or hydrodisulfide and assigned to be nitrosopersulfide (perthionitrite, SSNO; λ 412nm in aqueous buffers, pH 7.4; 448nm in DMF). Time-resolved chemiluminescence and UV-visible spectroscopy analyses suggest that its generation is preceded by formation of the short-lived NO-donor, thionitrite (SNO). In contrast to the latter, SSNO is rather stable at physiological pH and generates both NO and polysulfides on decomposition, resulting in sustained potentiation of SNAP-induced sGC stimulation. Thus, sulfide reacts with nitrosothiols to form multiple bioactive products; SSNO rather than SNO may account for some of the longer-lived effects of nitrosothiols and contribute to sulfide and NO signaling. | |
dc.format.extent | 11 | |
dc.format.extent | 1937145 | |
dc.language.iso | eng | |
dc.relation.ispartof | Redox Biology | en |
dc.subject | Hydrogen sulfide | en |
dc.subject | Nitric oxide | en |
dc.subject | Polysulfides | en |
dc.subject | cGMP | en |
dc.subject | HSNO | en |
dc.subject | Nitroxyl | en |
dc.subject | QH301 Biology | en |
dc.subject.lcc | QH301 | en |
dc.title | Nitrosopersulfide (SSNO) accounts for sustained NO bioactivity of S-nitrosothiols following reaction with sulfide | en |
dc.type | Journal article | en |
dc.contributor.institution | University of St Andrews. University of St Andrews | en |
dc.contributor.institution | University of St Andrews. School of Medicine | en |
dc.identifier.doi | 10.1016/j.redox.2013.12.031 | |
dc.description.status | Peer reviewed | en |
dc.identifier.url | http://www.scopus.com/inward/record.url?eid=2-s2.0-84893371806&partnerID=8YFLogxK | en |
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