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dc.contributor.authorSiggins, Alma
dc.contributor.authorEnright, Anne-Marie
dc.contributor.authorAbram, Florence
dc.contributor.authorBotting, Catherine
dc.contributor.authorO'Flaherty, Vincent
dc.identifier.citationSiggins , A , Enright , A-M , Abram , F , Botting , C & O'Flaherty , V 2012 , ' Impact of trichloroethylene exposure on the microbial diversity and protein expression in anaerobic granular biomass at 37 degrees C and 15 degrees C ' , Archaea .
dc.identifier.otherPURE: 41483177
dc.identifier.otherPURE UUID: dff2d2fd-2d14-47ba-b4b0-a9b42c28429c
dc.identifier.otherWOS: 000311169000001
dc.identifier.otherScopus: 84870015415
dc.description.abstractGranular biomass from a laboratory-scale anaerobic bioreactor trial was analysed to identify changes in microbial community structure and function in response to temperature and trichloroethylene (TCE). Two bioreactors were operated at 37 degrees C, while two were operated at 15 degrees C. At the time of sampling, one of each temperature pair of bioreactors was exposed to process failure-inducing concentrations of TCE (60 mg L-1) while the other served as a TCE-free control. Bacterial community structure was investigated using denaturing gradient gel electrophoresis (DGGE) and 16S rRNA gene clone library analysis. Temperature was identified as an important factor for bacterial community composition, while minor differences were associated with trichloroethylene supplementation. Proteobacteria was the dominant phylum in all bioreactors, while clone library analysis revealed a higher proportion of Bacteroidetes-, Chloroflexi-, and Firmicutes-like clones at 15 degrees C than at 37 degrees C. Comparative metaproteomics in the presence and absence of TCE was carried out by two-dimensional gel electrophoresis (2-DGE), and 28 protein spots were identified, with putative functions related to cellular processes, including methanogenesis, glycolysis, the glyoxylate cycle, and the methyl malonyl pathway. A good agreement between metaproteomic species assignment and phylogenetic information was observed, with 10 of the identified proteins associated with members of the phylum Proteobacteria.
dc.rightsCopyright © 2012 Alma Siggins et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.en
dc.subjectQR Microbiologyen
dc.titleImpact of trichloroethylene exposure on the microbial diversity and protein expression in anaerobic granular biomass at 37 degrees C and 15 degrees Cen
dc.typeJournal articleen
dc.description.versionPublisher PDFen
dc.contributor.institutionUniversity of St Andrews.School of Chemistryen
dc.contributor.institutionUniversity of St Andrews.Biomedical Sciences Research Complexen
dc.contributor.institutionUniversity of St Andrews.EaSTCHEMen
dc.description.statusPeer revieweden

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