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dc.contributor.authorTetteh, Antonia Y.
dc.contributor.authorSun, Katherine H.
dc.contributor.authorHung, Chiu-Yueh
dc.contributor.authorKittur, Farooqahmed S.
dc.contributor.authorIbeanu, Gordon C.
dc.contributor.authorWilliams, Daniel
dc.contributor.authorXie, Jiahua
dc.date.accessioned2014-05-01T11:01:01Z
dc.date.available2014-05-01T11:01:01Z
dc.date.issued2014-04-15
dc.identifier115015092
dc.identifier80c0dd50-9f21-4991-8408-2687095323bf
dc.identifier84900011543
dc.identifier.citationTetteh , A Y , Sun , K H , Hung , C-Y , Kittur , F S , Ibeanu , G C , Williams , D & Xie , J 2014 , ' Transcriptional Response of Selenopolypeptide Genes and Selenocysteine Biosynthesis Machinery Genes in Escherichia coli during Selenite Reduction ' , International Journal of Microbiology , vol. 2014 , 394835 . https://doi.org/10.1155/2014/394835en
dc.identifier.issn1687-918X
dc.identifier.otherBibtex: urn:3463362adf5b19a663e15020b3cb3589
dc.identifier.urihttps://hdl.handle.net/10023/4682
dc.descriptionThis work was supported by a United States Department of Agriculture-Cooperative State Research, Education, and Extension Service grant (no. 2009-35318-05032), a Biotechnology Research grant (no. 2007-BRG-1223) from the North Carolina Biotechnology Center, and a startup fund from the Golden LEAF Foundation to the Biomanufacturing Research Institute and Technology Enterprise (BRITE).en
dc.description.abstractBacteria can reduce toxic selenite into less toxic, elemental selenium (Se0), but the mechanism on how bacterial cells reduce selenite at molecular level is still not clear. We used Escherichia coli strain K12, a common bacterial strain, as a model to study its growth response to sodium selenite (Na2SeO3) treatment and then used quantitative real-time PCR (qRT-PCR) to quantify transcript levels of three E. coli selenopolypeptide genes and a set of machinery genes for selenocysteine (SeCys) biosynthesis and incorporation into polypeptides, whose involvements in the selenite reduction are largely unknown. We determined that 5 mM Na2SeO3 treatment inhibited growth by ∼50% while 0.001 to 0.01 mM treatments stimulated cell growth by ∼30%. Under 50% inhibitory or 30% stimulatory Na2SeO3 concentration, selenopolypeptide genes (fdnG, fdoG, and fdhF) whose products require SeCys but not SeCys biosynthesis machinery genes were found to be induced ≥2-fold. In addition, one sulfur (S) metabolic gene iscS and two previously reported selenite-responsive genes sodA and gutS were also induced ≥2-fold under 50% inhibitory concentration. Our findings provide insight about the detoxification of selenite in E. coli via induction of these genes involved in the selenite reduction process.
dc.format.extent11
dc.format.extent2068883
dc.language.isoeng
dc.relation.ispartofInternational Journal of Microbiologyen
dc.subjectQR Microbiologyen
dc.subject.lccQRen
dc.titleTranscriptional Response of Selenopolypeptide Genes and Selenocysteine Biosynthesis Machinery Genes in Escherichia coli during Selenite Reductionen
dc.typeJournal articleen
dc.contributor.institutionUniversity of St Andrews. School of Geography and Geosciencesen
dc.contributor.institutionUniversity of St Andrews. School of Earth & Environmental Sciencesen
dc.identifier.doi10.1155/2014/394835
dc.description.statusPeer revieweden
dc.identifier.urlhttp://dx.doi.org/10.1155/2014/394835en


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