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dc.contributor.authorTumapa, Sarinna
dc.contributor.authorHolden, Matthew T. G.
dc.contributor.authorVesaratchavest, Mongkol
dc.contributor.authorWuthiekanun, Vanaporn
dc.contributor.authorLimmathurotsakul, Direk
dc.contributor.authorChierakul, Wirongrong
dc.contributor.authorFeil, Edward J.
dc.contributor.authorCurrie, Bart J.
dc.contributor.authorDay, Nicholas P. J.
dc.contributor.authorNierman, William C.
dc.contributor.authorPeacock, Sharon J.
dc.date.accessioned2014-04-28T15:31:02Z
dc.date.available2014-04-28T15:31:02Z
dc.date.issued2008-04-25
dc.identifier.citationTumapa , S , Holden , M T G , Vesaratchavest , M , Wuthiekanun , V , Limmathurotsakul , D , Chierakul , W , Feil , E J , Currie , B J , Day , N P J , Nierman , W C & Peacock , S J 2008 , ' Burkholderia pseudomallei genome plasticity associated with genomic island variation ' BMC Genomics , vol. 9 , 190 . DOI: 10.1186/1471-2164-9-190en
dc.identifier.issn1471-2164
dc.identifier.otherPURE: 91775571
dc.identifier.otherPURE UUID: 4a887df8-8c40-419a-953a-4ef12c0ac67b
dc.identifier.otherWOS: 000256398500001
dc.identifier.otherScopus: 43849102127
dc.identifier.urihttp://hdl.handle.net/10023/4641
dc.descriptionSJP was supported by a Wellcome Trust Career Development Award in Clinical Tropical Medicine. This study was funded by the Wellcome Trust.en
dc.description.abstractBackground: Burkholderia pseudomallei is a soil-dwelling saprophyte and the cause of melioidosis. Horizontal gene transfer contributes to the genetic diversity of this pathogen and may be an important determinant of virulence potential. The genome contains genomic island (GI) regions that encode a broad array of functions. Although there is some evidence for the variable distribution of genomic islands in B. pseudomallei isolates, little is known about the extent of variation between related strains or their association with disease or environmental survival. Results: Five islands from B. pseudomallei strain K96243 were chosen as representatives of different types of genomic islands present in this strain, and their presence investigated in other B. pseudomallei. In silico analysis of 10 B. pseudomallei genome sequences provided evidence for the variable presence of these regions, together with micro-evolutionary changes that generate GI diversity. The diversity of GIs in 186 isolates from NE Thailand (83 environmental and 103 clinical isolates) was investigated using multiplex PCR screening. The proportion of all isolates positive by PCR ranged from 12% for a prophage-like island (GI 9), to 76% for a metabolic island (GI 16). The presence of each of the five GIs did not differ between environmental and disease-associated isolates (p > 0.05 for all five islands). The cumulative number of GIs per isolate for the 186 isolates ranged from 0 to 5 (median 2, IQR 1 to 3). The distribution of cumulative GI number did not differ between environmental and disease-associated isolates (p = 0.27). The presence of GIs was defined for the three largest clones in this collection (each defined as a single sequence type, ST, by multilocus sequence typing); these were ST 70 (n = 15 isolates), ST 54 (n = 11), and ST 167 (n = 9). The rapid loss and/or acquisition of gene islands was observed within individual clones. Comparisons were drawn between isolates obtained from the environment and from patients with melioidosis in order to examine the role of genomic islands in virulence and clinical associations. There was no reproducible association between the individual or cumulative presence of five GIs and a range of clinical features in 103 patients with melioidosis. Conclusion: Horizontal gene transfer of mobile genetic elements can rapidly alter the gene repertoire of B. pseudomallei. This study confirms the utility of a range of approaches in defining the presence and significance of genomic variation in natural populations of B. pseudomallei.en
dc.format.extent10en
dc.language.isoeng
dc.relation.ispartofBMC Genomicsen
dc.rights© 2008 Tumapa et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly citeden
dc.subjectStaphylococcus-aureusen
dc.subjectNorthern Australiaen
dc.subjectCausative agenten
dc.subjectMelioidosisen
dc.subjectVirulenceen
dc.subjectEvolutionen
dc.subjectGenesen
dc.subjectToolen
dc.subjectQH426 Geneticsen
dc.subjectQL Zoologyen
dc.subject.lccQH426en
dc.subject.lccQLen
dc.titleBurkholderia pseudomallei genome plasticity associated with genomic island variationen
dc.typeJournal articleen
dc.description.versionPublisher PDFen
dc.contributor.institutionUniversity of St Andrews. School of Medicineen
dc.contributor.institutionUniversity of St Andrews. Infection Groupen
dc.contributor.institutionUniversity of St Andrews. Biomedical Sciences Research Complexen
dc.identifier.doihttps://doi.org/10.1186/1471-2164-9-190
dc.description.statusPeer revieweden


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