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Structure, substrate recognition and reactivity of Leishmania major mevalonate kinase

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sgraja2007bmcstructbiol20.pdf (1.638Mb)
Date
30/03/2007
Author
Sgraja, Tanja
Smith, Terry K
Hunter, William N.
Keywords
Human inherited disease
Methanococcus-jannaschii
Biosynthetic-pathway
Trypanosoma-brucei
Crystal-structure
Expression
Purification
Superfamily
Isoprenoids
Mechanism
QH301 Biology
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Abstract
Background: Isoprenoid precursor synthesis via the mevalonate route in humans and pathogenic trypanosomatids is an important metabolic pathway. There is however, only limited information available on the structure and reactivity of the component enzymes in trypanosomatids. Since isoprenoid biosynthesis is essential for trypanosomatid viability and may provide new targets for therapeutic intervention it is important to characterize the pathway components. Results: Putative mevalonate kinase encoding genes from Leishmania major (LmMK) and Trypanosoma brucei (TbMK) have been cloned, over-expressed in and proteins isolated from procyclic-form T. brucei. A highly sensitive radioactive assay was developed and shows ATP-dependent phosphorylation of mevalonate. Apo and (R)-mevalonate bound crystal structures of LmMK, from a bacterial expression system, have been determined to high resolution providing, for the first time, information concerning binding of mevalonate to an MK. The mevalonate binds in a deep cavity lined by highly conserved residues. His25 is key for binding and for discrimination of (R)-over (S)-mevalonate, with the main chain amide interacting with the C3 hydroxyl group of ( R)mevalonate, and the side chain contributing, together with Val202 and Thr283, to the construction of a hydrophobic binding site for the C3 methyl substituent. The C5 hydroxyl, where phosphorylation occurs, points towards catalytic residues, Lys18 and Asp155. The activity of LmMK was significantly reduced compared to MK from other species and we were unable to obtain ATP-binding data. Comparisons with the rat MK:ATP complex were used to investigate how this substrate might bind. In LmMK, helix alpha 2 and the preceding polypeptide adopt a conformation, not seen in related kinase structures, impeding access to the nucleotide triphosphate binding site suggesting that a conformational rearrangement is required to allow ATP binding. Conclusion: Our new structural information, consistent with data on homologous enzymes allows a detailed description of how mevalonate is recognized and positioned for catalysis in MK. The mevalonate-binding site is highly conserved yet the ATP-binding site is structurally distinct in LmMK. We are unable to provide a definitive explanation for the low activity of recombinant protein isolated from a bacterial expression system compared to material isolated from procyclic-form Trypanosoma brucei.
Citation
Sgraja , T , Smith , T K & Hunter , W N 2007 , ' Structure, substrate recognition and reactivity of Leishmania major mevalonate kinase ' , BMC Structural Biology , vol. 7 , 20 . https://doi.org/10.1186/1472-6807-7-20
Publication
BMC Structural Biology
Status
Peer reviewed
DOI
https://doi.org/10.1186/1472-6807-7-20
Type
Journal article
Rights
© 2007 Sgraja et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited
Description
This research was supported by the German Academic Exchange Service (DAAD), the Wellcome Trust (TKS and WNH as Trust Senior Research fellows), the Biotechnology and Biological Science Research Council (Structural Proteomics of Rational Targets) and the European Synchrotron Radiation Facility.
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  • University of St Andrews Research
URL
http://www.scopus.com/inward/record.url?scp=34247137519&partnerID=8YFLogxK
URI
http://hdl.handle.net/10023/4633

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