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dc.contributor.authorRobinson, Kelly Joanne
dc.contributor.authorHazon, Neil
dc.contributor.authorLonergan, Mike
dc.contributor.authorPomeroy, Patrick
dc.date.accessioned2014-03-26T17:01:03Z
dc.date.available2014-03-26T17:01:03Z
dc.date.issued2014-01
dc.identifier.citationRobinson , K J , Hazon , N , Lonergan , M & Pomeroy , P 2014 , ' Validation of an enzyme-linked immunoassay (ELISA) for plasma oxytocin in a novel mammal species reveals potential errors induced by sampling procedure ' , Journal of Neuroscience Methods , vol. 226 , pp. 73-79 . https://doi.org/10.1016/j.jneumeth.2014.01.019en
dc.identifier.issn0165-0270
dc.identifier.otherPURE: 98220849
dc.identifier.otherPURE UUID: 23d599b5-4f88-4237-a345-89189ed3e53e
dc.identifier.otherScopus: 84894491136
dc.identifier.otherORCID: /0000-0003-1603-5630/work/46569072
dc.identifier.otherWOS: 000333946600007
dc.identifier.urihttp://hdl.handle.net/10023/4541
dc.descriptionThis work was conducted as part of a PhD, which was funded by the Natural Environment Research Council (NERC), UK and by SMRU Marine, St Andrews, UK.en
dc.description.abstractBACKGROUND: The neuropeptide oxytocin is increasing the focus of many studies investigating human and animal social behaviours and diseases. However, interpretation and comparison of results is made difficult by a lack of consistent methodological approaches towards analysing this hormone. NEW METHOD: This study determined the sample collection and analysis protocols that cause the least amounts of protocol dependant variation in plasma oxytocin concentrations detected by ELISA. The effect of vacutainer type, sample extraction prior to analysis and capture and restraint protocol were investigated while validating an assay protocol for two novel species, grey seals (Halichoerus grypus) and harbour seals (Phoca vitulina). RESULTS: Where samples are extracted prior to analysis, vacutainer type (EDTA mean: 8.25±0.56pg/ml, heparin mean: 8.25±0.62pg/ml, p=0.82), time taken to obtain a sample and restraint protocol did not affect the concentration of oxytocin detected. However, concentrations of oxytocin detected in raw plasma samples were significantly higher than those in extracted samples, and varied significantly with vacutainer type (EDTA mean: 534.4±43.7pg/ml, heparin mean: 300.9±19.6pg/ml, p<0.001) and capture and restraint methodology. There was no relationship between oxytocin concentrations detected in raw and extracted plasma (p=0.25). COMPARISON WITH EXISTING METHOD(S): Over half the reviewed published studies analysing plasma oxytocin use raw plasma and different vacutainer types are used without consistency or justification through-out the literature. CONCLUSIONS: We caution that studies using raw plasma are likely to over estimate oxytocin concentrations, cannot be used to accurately infer true values via correlations and are susceptible to variation according vacutainer type.
dc.language.isoeng
dc.relation.ispartofJournal of Neuroscience Methodsen
dc.rights© 2014, the Authors. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.en
dc.subjectEnzyme-linked immunoassay (ELISA)en
dc.subjectPlasma oxytocinen
dc.subjectRaw plasmaen
dc.subjectGrey seals (Halichoerus grypus)en
dc.subjectHarbour seals (Phoca vitulina)en
dc.subjectEDTAen
dc.subjectHeparinen
dc.subjectNERCen
dc.titleValidation of an enzyme-linked immunoassay (ELISA) for plasma oxytocin in a novel mammal species reveals potential errors induced by sampling procedureen
dc.typeJournal articleen
dc.description.versionPublisher PDFen
dc.contributor.institutionUniversity of St Andrews.School of Biologyen
dc.contributor.institutionUniversity of St Andrews.Marine Alliance for Science & Technology Scotlanden
dc.contributor.institutionUniversity of St Andrews.Scottish Oceans Instituteen
dc.contributor.institutionUniversity of St Andrews.Sea Mammal Research Uniten
dc.contributor.institutionUniversity of St Andrews.Centre for Research into Ecological & Environmental Modellingen
dc.identifier.doihttps://doi.org/10.1016/j.jneumeth.2014.01.019
dc.description.statusPeer revieweden


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