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dc.contributor.authorPraveen, Bavishna B.
dc.contributor.authorMazilu, Michael
dc.contributor.authorMarchington, Robert F.
dc.contributor.authorHerrington, C. Simon
dc.contributor.authorRiches, Andrew
dc.contributor.authorDholakia, Kishan
dc.identifier.citationPraveen , B B , Mazilu , M , Marchington , R F , Herrington , C S , Riches , A & Dholakia , K 2013 , ' Optimisation of wavelength modulated Raman spectroscopy : towards high throughput cell screening ' , PLoS One , vol. 8 , no. 6 , e67211 .
dc.identifier.otherPURE: 103066397
dc.identifier.otherPURE UUID: 907cb701-a003-4323-bb83-c0de406d72aa
dc.identifier.otherWOS: 000321223000091
dc.identifier.otherScopus: 84879381652
dc.description.abstractIn the field of biomedicine, Raman spectroscopy is a powerful technique to discriminate between normal and cancerous cells. However the strong background signal from the sample and the instrumentation affects the efficiency of this discrimination technique. Wavelength Modulated Raman spectroscopy (WMRS) may suppress the background from the Raman spectra. In this study we demonstrate a systematic approach for optimizing the various parameters of WMRS to achieve a reduction in the acquisition time for potential applications such as higher throughput cell screening. The Signal to Noise Ratio (SNR) of the Raman bands depends on the modulation amplitude, time constant and total acquisition time. It was observed that the sampling rate does not influence the signal to noise ratio of the Raman bands if three or more wavelengths are sampled. With these optimised WMRS parameters, we increased the throughput in the binary classification of normal human urothelial cells and bladder cancer cells by reducing the total acquisition time to 6 s which is significantly lower in comparison to previous acquisition times required for the discrimination between similar cell types.
dc.relation.ispartofPLoS Oneen
dc.rightsCopyright © 2013 Praveen et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.en
dc.subjectAbsorption spectroscopyen
dc.subjectWavelength Modulated Raman spectroscopy (WMRS)en
dc.subjectCell screeningen
dc.subjectSignal to Noise Ratio (SNR)en
dc.subjectUrothelial cellsen
dc.subjectBladder cancer cellsen
dc.subjectSDG 3 - Good Health and Well-beingen
dc.titleOptimisation of wavelength modulated Raman spectroscopy : towards high throughput cell screeningen
dc.typeJournal articleen
dc.description.versionPublisher PDFen
dc.contributor.institutionUniversity of St Andrews. School of Physics and Astronomyen
dc.contributor.institutionUniversity of St Andrews. School of Medicineen
dc.contributor.institutionUniversity of St Andrews. Biomedical Sciences Research Complexen
dc.description.statusPeer revieweden

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