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dc.contributor.authorBoonsilp, Siriphan
dc.contributor.authorThaipadungpanit, Janjira
dc.contributor.authorAmornchai, Premjit
dc.contributor.authorWuthiekanun, Vanaporn
dc.contributor.authorBailey, Mark S.
dc.contributor.authorHolden, Matthew
dc.contributor.authorZhang, Cuicai
dc.contributor.authorJiang, Xiugao
dc.contributor.authorKoizumi, Nobuo
dc.contributor.authorTaylor, Kyle
dc.contributor.authorGalloway, Renee
dc.contributor.authorHoffmaster, Alex R.
dc.contributor.authorCraig, Scott
dc.contributor.authorSmythe, Lee D.
dc.contributor.authorHartskeerl, Rudy A.
dc.contributor.authorDay, Nicholas P.
dc.contributor.authorChantratita, Narisara
dc.contributor.authorFeil, Edward J.
dc.contributor.authorAanensen, David M.
dc.contributor.authorSpratt, Brian G.
dc.contributor.authorPeacock, Sharon J.
dc.date.accessioned2014-03-12T16:01:02Z
dc.date.available2014-03-12T16:01:02Z
dc.date.issued2013-01
dc.identifier.citationBoonsilp , S , Thaipadungpanit , J , Amornchai , P , Wuthiekanun , V , Bailey , M S , Holden , M , Zhang , C , Jiang , X , Koizumi , N , Taylor , K , Galloway , R , Hoffmaster , A R , Craig , S , Smythe , L D , Hartskeerl , R A , Day , N P , Chantratita , N , Feil , E J , Aanensen , D M , Spratt , B G & Peacock , S J 2013 , ' A Single Multilocus Sequence Typing (MLST) scheme for seven pathogenic Leptospira Species ' , PLoS Neglected Tropical Diseases , vol. 7 , no. 1 , e1954 . https://doi.org/10.1371/journal.pntd.0001954en
dc.identifier.issn1935-2735
dc.identifier.otherPURE: 91750562
dc.identifier.otherPURE UUID: 9084975e-1116-4dea-a7a7-d18272217334
dc.identifier.otherWOS: 000314360200005
dc.identifier.otherScopus: 84873493253
dc.identifier.otherORCID: /0000-0002-4958-2166/work/60196408
dc.identifier.urihttp://hdl.handle.net/10023/4519
dc.descriptionThis study was funded by the Wellcome Trust. BGS and DMA were funded by Wellcome Trust grant 089472. SJP receives support from the NIHR Cambridge Biomedical Research Centreen
dc.description.abstractBackground: The available Leptospira multilocus sequence typing (MLST) scheme supported by a MLST website is limited to L. interrogans and L. kirschneri. Our aim was to broaden the utility of this scheme to incorporate a total of seven pathogenic species. Methodology and Findings: We modified the existing scheme by replacing one of the seven MLST loci (fadD was changed to caiB), as the former gene did not appear to be present in some pathogenic species. Comparison of the original and modified schemes using data for L. interrogans and L. kirschneri demonstrated that the discriminatory power of the two schemes was not significantly different. The modified scheme was used to further characterize 325 isolates (L. alexanderi [n = 5], L. borgpetersenii [n = 34], L. interrogans [n = 222], L. kirschneri [n = 29], L. noguchii [n = 9], L. santarosai [n = 10], and L. weilii [n = 16]). Phylogenetic analysis using concatenated sequences of the 7 loci demonstrated that each species corresponded to a discrete clade, and that no strains were misclassified at the species level. Comparison between genotype and serovar was possible for 254 isolates. Of the 31 sequence types (STs) represented by at least two isolates, 18 STs included isolates assigned to two or three different serovars. Conversely, 14 serovars were identified that contained between 2 to 10 different STs. New observations were made on the global phylogeography of Leptospira spp., and the utility of MLST in making associations between human disease and specific maintenance hosts was demonstrated. Conclusion: The new MLST scheme, supported by an updated MLST website, allows the characterization and species assignment of isolates of the seven major pathogenic species associated with leptospirosis.
dc.format.extent10
dc.language.isoeng
dc.relation.ispartofPLoS Neglected Tropical Diseasesen
dc.rightsThis is an open-access article, free of all copyright, and may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose. The work is made available under the Creative Commons CC0 public domain dedication.en
dc.subjectGenetic diversityen
dc.subjectInterrogansen
dc.subjectEpidemiologyen
dc.subjectRelatednessen
dc.subjectQH301 Biologyen
dc.subject.lccQH301en
dc.titleA Single Multilocus Sequence Typing (MLST) scheme for seven pathogenic Leptospira Speciesen
dc.typeJournal articleen
dc.description.versionPublisher PDFen
dc.contributor.institutionUniversity of St Andrews.School of Medicineen
dc.contributor.institutionUniversity of St Andrews.Infection Groupen
dc.contributor.institutionUniversity of St Andrews.Biomedical Sciences Research Complexen
dc.identifier.doihttps://doi.org/10.1371/journal.pntd.0001954
dc.description.statusPeer revieweden


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