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Virulent and avirulent strains of Toxoplasma gondii which differ in their glycosylphosphatidylinositol content induce similar biological functions in macrophages
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dc.contributor.author | Niehus, Sebastian | |
dc.contributor.author | Smith, Terry K | |
dc.contributor.author | Azzouz, Nahid | |
dc.contributor.author | Campos, Marco A | |
dc.contributor.author | Dubremetz , Jean-François | |
dc.contributor.author | Gazzinelli , Ricardo T | |
dc.contributor.author | Schwarz, Ralph T | |
dc.contributor.author | Debierre-Grockiego, Françoise | |
dc.date.accessioned | 2014-02-25T17:31:01Z | |
dc.date.available | 2014-02-25T17:31:01Z | |
dc.date.issued | 2014-01-28 | |
dc.identifier.citation | Niehus , S , Smith , T K , Azzouz , N , Campos , M A , Dubremetz , J-F , Gazzinelli , R T , Schwarz , R T & Debierre-Grockiego , F 2014 , ' Virulent and avirulent strains of Toxoplasma gondii which differ in their glycosylphosphatidylinositol content induce similar biological functions in macrophages ' , PLoS One , vol. 9 , no. 1 , e85386 . https://doi.org/10.1371/journal.pone.0085386 | en |
dc.identifier.issn | 1932-6203 | |
dc.identifier.other | PURE: 99454045 | |
dc.identifier.other | PURE UUID: 51f997ba-fc36-47de-93cf-8702d3df4383 | |
dc.identifier.other | PubMed: 24489660 | |
dc.identifier.other | WOS: 000330510000013 | |
dc.identifier.other | Scopus: 84900314374 | |
dc.identifier.uri | https://hdl.handle.net/10023/4465 | |
dc.description.abstract | Glycosylphosphatidylinositols (GPIs) from several protozoan parasites are thought to elicit a detrimental stimulation of the host innate immune system aside their main function to anchor surface proteins. Here we analyzed the GPI biosynthesis of an avirulent Toxoplasma gondii type 2 strain (PTG) by metabolic radioactive labeling. We determined the biological function of individual GPI species in the PTG strain in comparison with previously characterized GPI-anchors of a virulent strain (RH). The GPI intermediates of both strains were structurally similar, however the abundance of two of six GPI intermediates was significantly reduced in the PTG strain. The side-by-side comparison of GPI-anchor content revealed that the PTG strain had only ~34% of the protein-free GPIs as well as ~70% of the GPI-anchored proteins with significantly lower rates of protein N-glycosylation compared to the RH strain. All mature GPIs from both strains induced comparable secretion levels of TNF-α and IL-12p40, and initiated TLR4/MyD88-dependent NF-κBp65 activation in macrophages. Taken together, these results demonstrate that PTG and RH strains differ in their GPI biosynthesis and possess significantly different GPI-anchor content, while individual GPI species of both strains induce similar biological functions in macrophages. Figures | |
dc.language.iso | eng | |
dc.relation.ispartof | PLoS One | en |
dc.rights | © 2014 Niehus et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. | en |
dc.subject | QR355 Virology | en |
dc.subject.lcc | QR355 | en |
dc.title | Virulent and avirulent strains of Toxoplasma gondii which differ in their glycosylphosphatidylinositol content induce similar biological functions in macrophages | en |
dc.type | Journal article | en |
dc.contributor.sponsor | The Wellcome Trust | en |
dc.description.version | Publisher PDF | en |
dc.contributor.institution | University of St Andrews. School of Biology | en |
dc.contributor.institution | University of St Andrews. Biomedical Sciences Research Complex | en |
dc.identifier.doi | https://doi.org/10.1371/journal.pone.0085386 | |
dc.description.status | Peer reviewed | en |
dc.identifier.grantnumber | 093228/Z/10/Z | en |
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