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dc.contributor.authorAntkowiak, Maciej
dc.contributor.authorTorres, Maria Leilani
dc.contributor.authorWitts, Emily Charlotte
dc.contributor.authorMiles, Gareth Brian
dc.contributor.authorDholakia, Kishan
dc.contributor.authorGunn-Moore, Frank J
dc.date.accessioned2014-01-21T16:31:05Z
dc.date.available2014-01-21T16:31:05Z
dc.date.issued2013-11-21
dc.identifier.citationAntkowiak , M , Torres , M L , Witts , E C , Miles , G B , Dholakia , K & Gunn-Moore , F J 2013 , ' Fast targeted gene transfection and optogenetic modification of single neurons using femtosecond laser irradiation ' , Scientific Reports , vol. 3 , 3281 . https://doi.org/10.1038/srep03281en
dc.identifier.issn2045-2322
dc.identifier.otherPURE: 74492608
dc.identifier.otherPURE UUID: fbb3fd8e-fb80-4287-ba92-fc33791fba69
dc.identifier.otherScopus: 84888233190
dc.identifier.otherORCID: /0000-0003-3422-3387/work/34730433
dc.identifier.otherORCID: /0000-0002-8624-4625/work/29135007
dc.identifier.urihttp://hdl.handle.net/10023/4411
dc.descriptionThis work is supported by the UK Engineering Physical Sciences Research Council (EPSRC).en
dc.description.abstractA prevailing problem in neuroscience is the fast and targeted delivery of DNA into selected neurons. The development of an appropriate methodology would enable the transfection of multiple genes into the same cell or different genes into different neighboring cells as well as rapid cell selective functionalization of neurons. Here, we show that optimized femtosecond optical transfection fulfills these requirements. We also demonstrate successful optical transfection of channelrhodopsin-2 in single selected neurons. We extend the functionality of this technique for wider uptake by neuroscientists by using fast three-dimensional laser beam steering enabling an image-guided “point-and-transfect” user-friendly transfection of selected cells. A sub-second transfection timescale per cell makes this method more rapid by at least two orders of magnitude when compared to alternative single-cell transfection techniques. This novel technology provides the ability to carry out large-scale cell selective genetic studies on neuronal ensembles and perform rapid genetic programming of neural circuits.
dc.format.extent8
dc.language.isoeng
dc.relation.ispartofScientific Reportsen
dc.rights(c) The authors. This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 3.0 Unported License. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-sa/3.0/en
dc.subjectMolecular neuroscienceen
dc.subjectCellular neuroscienceen
dc.subjectGenetic transductionen
dc.subjectBiophotonicsen
dc.subjectRC0321 Neuroscience. Biological psychiatry. Neuropsychiatryen
dc.subject.lccRC0321en
dc.titleFast targeted gene transfection and optogenetic modification of single neurons using femtosecond laser irradiationen
dc.typeJournal articleen
dc.description.versionPublisher PDFen
dc.contributor.institutionUniversity of St Andrews.School of Medicineen
dc.contributor.institutionUniversity of St Andrews.School of Psychology and Neuroscienceen
dc.contributor.institutionUniversity of St Andrews.Institute of Behavioural and Neural Sciencesen
dc.contributor.institutionUniversity of St Andrews.School of Physics and Astronomyen
dc.contributor.institutionUniversity of St Andrews.School of Biologyen
dc.contributor.institutionUniversity of St Andrews.Biomedical Sciences Research Complexen
dc.identifier.doihttps://doi.org/10.1038/srep03281
dc.description.statusPeer revieweden


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