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Fast targeted gene transfection and optogenetic modification of single neurons using femtosecond laser irradiation
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dc.contributor.author | Antkowiak, Maciej | |
dc.contributor.author | Torres, Maria Leilani | |
dc.contributor.author | Witts, Emily Charlotte | |
dc.contributor.author | Miles, Gareth Brian | |
dc.contributor.author | Dholakia, Kishan | |
dc.contributor.author | Gunn-Moore, Frank J | |
dc.date.accessioned | 2014-01-21T16:31:05Z | |
dc.date.available | 2014-01-21T16:31:05Z | |
dc.date.issued | 2013-11-21 | |
dc.identifier | 74492608 | |
dc.identifier | fbb3fd8e-fb80-4287-ba92-fc33791fba69 | |
dc.identifier | 84888233190 | |
dc.identifier.citation | Antkowiak , M , Torres , M L , Witts , E C , Miles , G B , Dholakia , K & Gunn-Moore , F J 2013 , ' Fast targeted gene transfection and optogenetic modification of single neurons using femtosecond laser irradiation ' , Scientific Reports , vol. 3 , 3281 . https://doi.org/10.1038/srep03281 | en |
dc.identifier.issn | 2045-2322 | |
dc.identifier.other | ORCID: /0000-0003-3422-3387/work/34730433 | |
dc.identifier.other | ORCID: /0000-0002-8624-4625/work/29135007 | |
dc.identifier.uri | https://hdl.handle.net/10023/4411 | |
dc.description | This work is supported by the UK Engineering Physical Sciences Research Council (EPSRC). | en |
dc.description.abstract | A prevailing problem in neuroscience is the fast and targeted delivery of DNA into selected neurons. The development of an appropriate methodology would enable the transfection of multiple genes into the same cell or different genes into different neighboring cells as well as rapid cell selective functionalization of neurons. Here, we show that optimized femtosecond optical transfection fulfills these requirements. We also demonstrate successful optical transfection of channelrhodopsin-2 in single selected neurons. We extend the functionality of this technique for wider uptake by neuroscientists by using fast three-dimensional laser beam steering enabling an image-guided “point-and-transfect” user-friendly transfection of selected cells. A sub-second transfection timescale per cell makes this method more rapid by at least two orders of magnitude when compared to alternative single-cell transfection techniques. This novel technology provides the ability to carry out large-scale cell selective genetic studies on neuronal ensembles and perform rapid genetic programming of neural circuits. | |
dc.format.extent | 8 | |
dc.format.extent | 792531 | |
dc.language.iso | eng | |
dc.relation.ispartof | Scientific Reports | en |
dc.subject | Molecular neuroscience | en |
dc.subject | Cellular neuroscience | en |
dc.subject | Genetic transduction | en |
dc.subject | Biophotonics | en |
dc.subject | RC0321 Neuroscience. Biological psychiatry. Neuropsychiatry | en |
dc.subject.lcc | RC0321 | en |
dc.title | Fast targeted gene transfection and optogenetic modification of single neurons using femtosecond laser irradiation | en |
dc.type | Journal article | en |
dc.contributor.sponsor | EPSRC | en |
dc.contributor.institution | University of St Andrews. School of Medicine | en |
dc.contributor.institution | University of St Andrews. School of Psychology and Neuroscience | en |
dc.contributor.institution | University of St Andrews. Institute of Behavioural and Neural Sciences | en |
dc.contributor.institution | University of St Andrews. School of Physics and Astronomy | en |
dc.contributor.institution | University of St Andrews. School of Biology | en |
dc.contributor.institution | University of St Andrews. Biomedical Sciences Research Complex | en |
dc.identifier.doi | 10.1038/srep03281 | |
dc.description.status | Peer reviewed | en |
dc.identifier.grantnumber | EP/H045368/1 | en |
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