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dc.contributor.authorSomorjai, Ildiko M. L.
dc.contributor.authorMartinez-Arias, Alfonso
dc.date.accessioned2013-12-04T11:01:02Z
dc.date.available2013-12-04T11:01:02Z
dc.date.issued2008-08-06
dc.identifier.citationSomorjai , I M L & Martinez-Arias , A 2008 , ' Wingless signalling alters the levels, subcellular distribution and dynamics of armadillo and E-Cadherin in third instar larval wing imaginal discs ' , PLoS One , vol. 3 , no. 8 , e2893 . https://doi.org/10.1371/journal.pone.0002893en
dc.identifier.issn1932-6203
dc.identifier.otherPURE: 34020274
dc.identifier.otherPURE UUID: 63a9cdff-94e8-4bcf-b41f-ecbe20089a2a
dc.identifier.otherWOS: 000264366600049
dc.identifier.otherScopus: 51449094367
dc.identifier.otherORCID: /0000-0001-5243-6664/work/46168683
dc.identifier.urihttps://hdl.handle.net/10023/4253
dc.description.abstractBackground: Armadillo, the Drosophila orthologue of vertebrate beta-catenin, plays a dual role as the key effector of Wingless/Wnt1 signalling, and as a bridge between E-Cadherin and the actin cytoskeleton. In the absence of ligand, Armadillo is phosphorylated and targeted to the proteasome. Upon binding of Wg to its receptors, the "degradation complex'' is inhibited; Armadillo is stabilised and enters the nucleus to transcribe targets. Methodology/Principal Findings: Although the relationship between signalling and adhesion has been extensively studied, few in vivo data exist concerning how the "transcriptional'' and "adhesive'' pools of Armadillo are regulated to orchestrate development. We have therefore addressed how the subcellular distribution of Armadillo and its association with E-Cadherin change in larval wing imaginal discs, under wild type conditions and upon signalling. Using confocal microscopy, we show that Armadillo and E-Cadherin are spatio-temporally regulated during development, and that a punctate species becomes concentrated in a subapical compartment in response to Wingless. In order to further dissect this phenomenon, we overexpressed Armadillo mutants exhibiting different levels of activity and stability, but retaining E-Cadherin binding. Arm(S10) displaces endogenous Armadillo from the AJ and the basolateral membrane, while leaving E-Cadherin relatively undisturbed. Surprisingly, Delta NArm(1-155) caused displacement of both Armadillo and E-Cadherin, results supported by our novel method of quantification. However, only membrane-targeted Myr-Delta NArm(1-155) produced comparable nuclear accumulation of Armadillo and signalling to Arm(S10). These experiments also highlighted a row of cells at the A/P boundary depleted of E-Cadherin at the AJ, but containing actin. Conclusions/Significance: Taken together, our results provide in vivo evidence for a complex non-linear relationship between Armadillo levels, subcellular distribution and Wingless signalling. Moreover, this study highlights the importance of Armadillo in regulating the subcellular distribution of E-Cadherin
dc.format.extent19
dc.language.isoeng
dc.relation.ispartofPLoS Oneen
dc.rights© 2008 Somorjai et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.en
dc.subjectArmadillosen
dc.subjectWingless signallingen
dc.subjectSubcellular distributionen
dc.subjectE-Cadherinen
dc.titleWingless signalling alters the levels, subcellular distribution and dynamics of armadillo and E-Cadherin in third instar larval wing imaginal discsen
dc.typeJournal articleen
dc.description.versionPublisher PDFen
dc.contributor.institutionUniversity of St Andrews. School of Biologyen
dc.contributor.institutionUniversity of St Andrews. Marine Alliance for Science & Technology Scotlanden
dc.contributor.institutionUniversity of St Andrews. Biomedical Sciences Research Complexen
dc.identifier.doihttps://doi.org/10.1371/journal.pone.0002893
dc.description.statusPeer revieweden


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