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dc.contributor.authorVijayakrishnan, Swetha
dc.contributor.authorLoney, Colin
dc.contributor.authorJackson, David
dc.contributor.authorSuphamungmee, Worawit
dc.contributor.authorRixon, Frazer J.
dc.contributor.authorBhella, David
dc.identifier.citationVijayakrishnan , S , Loney , C , Jackson , D , Suphamungmee , W , Rixon , F J & Bhella , D 2013 , ' Cryotomography of budding influenza A virus reveals filaments with diverse morphologies that mostly do not bear a genome at their distal end ' , PLoS Pathogens , vol. 9 , no. 6 , e1003413 .
dc.identifier.otherPURE: 77172071
dc.identifier.otherPURE UUID: 52b9ee1a-138e-4fa3-a858-961761ce1faf
dc.identifier.otherWOS: 000321206600025
dc.identifier.otherScopus: 84879526764
dc.descriptionThis research was funded by the UK Medical Research Council - core funding to the MRC Centre for Virus Research (
dc.description.abstractInfluenza viruses exhibit striking variations in particle morphology between strains. Clinical isolates of influenza A virus have been shown to produce long filamentous particles while laboratory-adapted strains are predominantly spherical. However, the role of the filamentous phenotype in the influenza virus infectious cycle remains undetermined. We used cryo-electron tomography to conduct the first three-dimensional study of filamentous virus ultrastructure in particles budding from infected cells. Filaments were often longer than 10 microns and sometimes had bulbous heads at their leading ends, some of which contained tubules we attribute to M1 while none had recognisable ribonucleoprotein (RNP) and hence genome segments. Long filaments that did not have bulbs were infrequently seen to bear an ordered complement of RNPs at their distal ends. Imaging of purified virus also revealed diverse filament morphologies; short rods (bacilliform virions) and longer filaments. Bacilliform virions contained an ordered complement of RNPs while longer filamentous particles were narrower and mostly appeared to lack this feature, but often contained fibrillar material along their entire length. The important ultrastructural differences between these diverse classes of particles raise the possibility of distinct morphogenetic pathways and functions during the infectious process.
dc.relation.ispartofPLoS Pathogensen
dc.rights© 2013 Vijayakrishnan et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.en
dc.subjectCryoelectron Tomographyen
dc.subjectMatrix proteinen
dc.subjectRibonucleoprotein complexesen
dc.subjectParelectron-microscopyticle formationen
dc.subjectRNA segmentsen
dc.subjectQH301 Biologyen
dc.titleCryotomography of budding influenza A virus reveals filaments with diverse morphologies that mostly do not bear a genome at their distal enden
dc.typeJournal articleen
dc.description.versionPublisher PDFen
dc.contributor.institutionUniversity of St Andrews.School of Biologyen
dc.description.statusPeer revieweden

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