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dc.contributor.authorZheng, Maozhong
dc.contributor.authorBeck, Martina
dc.contributor.authorMuller, Jens
dc.contributor.authorWang, Xiaohua
dc.contributor.authorWang, Qinli
dc.contributor.authorBaluska, Frantisek
dc.contributor.authorLogan, David
dc.contributor.authorSamaj, Jozef
dc.contributor.authorLin, Jinxing
dc.date.accessioned2013-12-02T10:01:01Z
dc.date.available2013-12-02T10:01:01Z
dc.date.issued2009-06
dc.identifier.citationZheng , M , Beck , M , Muller , J , Wang , X , Wang , Q , Baluska , F , Logan , D , Samaj , J & Lin , J 2009 , ' Actin turnover is required for myosin-dependent mitochondrial movements in Arabidopsis root hairs ' , PLoS ONE , vol. 4 , no. 6 , e5961 . https://doi.org/10.1371/journal.pone.0005961en
dc.identifier.issn1932-6203
dc.identifier.otherPURE: 406956
dc.identifier.otherPURE UUID: 5b77df04-587c-4009-8bed-37bc15330bf3
dc.identifier.otherstandrews_research_output: 23629
dc.identifier.otherScopus: 67650099504
dc.identifier.urihttps://hdl.handle.net/10023/4230
dc.description.abstractBackground: Previous studies have shown that plant mitochondrial movements are myosin-based along actin filaments, which undergo continuous turnover by the exchange of actin subunits from existing filaments. Although earlier studies revealed that actin filament dynamics are essential for many functions of the actin cytoskeleton, there are little data connecting actin dynamics and mitochondrial movements. Methodology/Principal Findings: We addressed the role of actin filament dynamics in the control of mitochondrial movements by treating cells with various pharmaceuticals that affect actin filament assembly and disassembly. Confocal microscopy of Arabidopsis thaliana root hairs expressing GFP-FABD2 as an actin filament reporter showed that mitochondrial distribution was in agreement with the arrangement of actin filaments in root hairs at different developmental stages. Analyses of mitochondrial trajectories and instantaneous velocities immediately following pharmacological perturbation of the cytoskeleton using variable-angle evanescent wave microscopy and/or spinning disk confocal microscopy revealed that mitochondrial velocities were regulated by myosin activity and actin filament dynamics. Furthermore, simultaneous visualization of mitochondria and actin filaments suggested that mitochondrial positioning might involve depolymerization of actin filaments on the surface of mitochondria. Conclusions/Significance: Base on these results we propose a mechanism for the regulation of mitochondrial speed of movements, positioning, and direction of movements that combines the coordinated activity of myosin and the rate of actin turnover, together with microtubule dynamics, which directs the positioning of actin polymerization events.
dc.language.isoeng
dc.relation.ispartofPLoS ONEen
dc.rights© 2009 Zheng et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.en
dc.subjectActinsen
dc.subjectMitochondriaen
dc.subjectArabidopsis thalianaen
dc.subjectRoot hairsen
dc.subjectQ Scienceen
dc.subject.lccQen
dc.titleActin turnover is required for myosin-dependent mitochondrial movements in Arabidopsis root hairsen
dc.typeJournal articleen
dc.description.versionPublisher PDFen
dc.contributor.institutionUniversity of St Andrews. School of Biologyen
dc.identifier.doihttps://doi.org/10.1371/journal.pone.0005961
dc.description.statusPeer revieweden
dc.identifier.urlhttp://www.scopus.com/inward/record.url?scp=67650099504&partnerID=8YFLogxKen


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