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dc.contributor.authorNiehuis, Oliver
dc.contributor.authorGibson, Joshua D.
dc.contributor.authorRosenberg, Michael S.
dc.contributor.authorPannebakker, Bart A.
dc.contributor.authorKoevoets, Tosca
dc.contributor.authorJudson, Andrea K.
dc.contributor.authorDesjardins, Christopher A.
dc.contributor.authorKennedy, Kathleen
dc.contributor.authorDuggan, David
dc.contributor.authorBeukeboom, Leo W.
dc.contributor.authorvan de Zande, Louis
dc.contributor.authorShuker, David Michael
dc.contributor.authorWerren, John H.
dc.contributor.authorGadau, Juergen
dc.identifier.citationNiehuis , O , Gibson , J D , Rosenberg , M S , Pannebakker , B A , Koevoets , T , Judson , A K , Desjardins , C A , Kennedy , K , Duggan , D , Beukeboom , L W , van de Zande , L , Shuker , D M , Werren , J H & Gadau , J 2010 , ' Recombination and its impact on the genome of the haplodiploid parasitoid wasp Nasonia ' , PLoS One , vol. 5 , no. 1 , e8597 .
dc.identifier.otherPURE: 2085554
dc.identifier.otherPURE UUID: 671eef68-4ad7-4fd9-9fc0-664894020599
dc.identifier.otherWOS: 000273778900001
dc.identifier.otherScopus: 77649312033
dc.description.abstractHomologous meiotic recombination occurs in most sexually reproducing organisms, yet its evolutionary advantages are elusive. Previous research explored recombination in the honeybee, a eusocial hymenopteran with an exceptionally high genome-wide recombination rate. A comparable study in a non-social member of the Hymenoptera that would disentangle the impact of sociality from Hymenoptera-specific features such as haplodiploidy on the evolution of the high genome-wide recombination rate in social Hymenoptera is missing. Utilizing single-nucleotide polymorphisms (SNPs) between two Nasonia parasitoid wasp genomes, we developed a SNP genotyping microarray to infer a high-density linkage map for Nasonia. The map comprises 1,255 markers with an average distance of 0.3 cM. The mapped markers enabled us to arrange 265 scaffolds of the Nasonia genome assembly 1.0 on the linkage map, representing 63.6% of the assembled N. vitripennis genome. We estimated a genome-wide recombination rate of 1.4-1.5 cM/Mb for Nasonia, which is less than one tenth of the rate reported for the honeybee. The local recombination rate in Nasonia is positively correlated with the distance to the center of the linkage groups, GC content, and the proportion of simple repeats. In contrast to the honeybee genome, gene density in the parasitoid wasp genome is positively associated with the recombination rate; regions of low recombination are characterized by fewer genes with larger introns and by a greater distance between genes. Finally, we found that genes in regions of the genome with a low recombination frequency tend to have a higher ratio of non-synonymous to synonymous substitutions, likely due to the accumulation of slightly deleterious non-synonymous substitutions. These findings are consistent with the hypothesis that recombination reduces interference between linked sites and thereby facilitates adaptive evolution and the purging of deleterious mutations. Our results imply that the genomes of haplodiploid and of diploid higher eukaryotes do not differ systematically in their recombination rates and associated parameters.
dc.relation.ispartofPLoS Oneen
dc.rights© 2010 Niehuis et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.en
dc.subjectSingle nucleotide polymorphismen
dc.subjectMaximum likelihood modelsen
dc.subjectX-chromosome centromereen
dc.subjectBiased gene conversionen
dc.subjectNatural selectionen
dc.subjectDrosophilia melanogasteren
dc.subjectMeiotic recombinationen
dc.subjectQ Scienceen
dc.titleRecombination and its impact on the genome of the haplodiploid parasitoid wasp Nasoniaen
dc.typeJournal articleen
dc.description.versionPublisher PDFen
dc.contributor.institutionUniversity of St Andrews.School of Biologyen
dc.contributor.institutionUniversity of St Andrews.Scottish Oceans Instituteen
dc.description.statusPeer revieweden

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