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dc.contributor.authorSeipke, Ryan F.
dc.contributor.authorBarke, Joerg
dc.contributor.authorBrearley, Charles
dc.contributor.authorHill, Lionel
dc.contributor.authorYu, Douglas W.
dc.contributor.authorGoss, Rebecca J. M.
dc.contributor.authorHutchings, Matthew I.
dc.date.accessioned2013-11-05T11:31:01Z
dc.date.available2013-11-05T11:31:01Z
dc.date.issued2011-08-03
dc.identifier.citationSeipke , R F , Barke , J , Brearley , C , Hill , L , Yu , D W , Goss , R J M & Hutchings , M I 2011 , ' A single Streptomyces symbiont makes multiple antifungals to support the fungus farming ant Acromyrmex octospinosus ' , PLoS ONE , vol. 6 , no. 8 , e22028 . https://doi.org/10.1371/journal.pone.0022028en
dc.identifier.issn1932-6203
dc.identifier.otherPURE: 50707226
dc.identifier.otherPURE UUID: 4e544e78-b8c0-497d-a5ec-6c6e655e8625
dc.identifier.otherWOS: 000293558900009
dc.identifier.otherScopus: 79961040245
dc.identifier.urihttp://hdl.handle.net/10023/4159
dc.description.abstractAttine ants are dependent on a cultivated fungus for food and use antibiotics produced by symbiotic Actinobacteria as weedkillers in their fungus gardens. Actinobacterial species belonging to the genera Pseudonocardia, Streptomyces and Amycolatopsis have been isolated from attine ant nests and shown to confer protection against a range of microfungal weeds. In previous work on the higher attine Acromyrmex octospinosus we isolated a Streptomyces strain that produces candicidin, consistent with another report that attine ants use Streptomyces-produced candicidin in their fungiculture. Here we report the genome analysis of this Streptomyces strain and identify multiple antibiotic biosynthetic pathways. We demonstrate, using gene disruptions and mass spectrometry, that this single strain has the capacity to make candicidin and multiple antimycin compounds. Although antimycins have been known for > 60 years we report the sequence of the biosynthetic gene cluster for the first time. Crucially, disrupting the candicidin and antimycin gene clusters in the same strain had no effect on bioactivity against a co-evolved nest pathogen called Escovopsis that has been identified in similar to 30% of attine ant nests. Since the Streptomyces strain has strong bioactivity against Escovopsis we conclude that it must make additional antifungal(s) to inhibit Escovopsis. However, candicidin and antimycins likely offer protection against other microfungal weeds that infect the attine fungal gardens. Thus, we propose that the selection of this biosynthetically prolific strain from the natural environment provides A. octospinosus with broad spectrum activity against Escovopsis and other microfungal weeds.
dc.format.extent8
dc.language.isoeng
dc.relation.ispartofPLoS ONEen
dc.rights© 2011 Seipke et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.en
dc.subjectBacteriaen
dc.subjectEscovopsisen
dc.subjectCloningen
dc.subjectMicrobe symbiosisen
dc.subjectGene clusteren
dc.subjectCoevolutionen
dc.subjectBiosynthesisen
dc.subjectLeaf-cutting antsen
dc.subjectQ Scienceen
dc.subject.lccQen
dc.titleA single Streptomyces symbiont makes multiple antifungals to support the fungus farming ant Acromyrmex octospinosusen
dc.typeJournal articleen
dc.description.versionPublisher PDFen
dc.contributor.institutionUniversity of St Andrews.School of Chemistryen
dc.contributor.institutionUniversity of St Andrews.EaSTCHEMen
dc.contributor.institutionUniversity of St Andrews.Biomedical Sciences Research Complexen
dc.identifier.doihttps://doi.org/10.1371/journal.pone.0022028
dc.description.statusPeer revieweden


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