Files in this item
Establishment of a reverse genetics system for Schmallenberg virus, a newly emerged orthobunyavirus in Europe
Item metadata
dc.contributor.author | Elliott, Richard M. | |
dc.contributor.author | Blakqori, Gjon | |
dc.contributor.author | van Knippenberg, Ingeborg C. | |
dc.contributor.author | Koudriakova, Elina | |
dc.contributor.author | Li, Ping | |
dc.contributor.author | McLees, Angela | |
dc.contributor.author | Shi, Xiaohong | |
dc.contributor.author | Szemiel, Agnieszka M. | |
dc.date.accessioned | 2013-08-05T12:31:01Z | |
dc.date.available | 2013-08-05T12:31:01Z | |
dc.date.issued | 2013-04 | |
dc.identifier | 62931874 | |
dc.identifier | ba907729-a6c5-45e4-a14c-6bf4efcbe703 | |
dc.identifier | 000317995200019 | |
dc.identifier | 84874062016 | |
dc.identifier.citation | Elliott , R M , Blakqori , G , van Knippenberg , I C , Koudriakova , E , Li , P , McLees , A , Shi , X & Szemiel , A M 2013 , ' Establishment of a reverse genetics system for Schmallenberg virus, a newly emerged orthobunyavirus in Europe ' , Journal of General Virology , vol. 94 , no. 4 , pp. 851-859 . https://doi.org/10.1099/vir.0.049981-0 | en |
dc.identifier.issn | 0022-1317 | |
dc.identifier.uri | https://hdl.handle.net/10023/3908 | |
dc.description.abstract | Schmallenberg virus (SBV) is a newly emerged orthobunyavirus that has caused widespread disease in cattle, sheep and goats in Europe. Like other orthobunyaviruses, SBV is characterized by a tripartite negative-sense RNA genome that encodes four structural and two non-structural proteins. This study showed that SBV has a wide in vitro host range, and that BHK-21 cells are a convenient host for both SBV propagation and assay by plaque titration. The SBV genome segments were cloned as cDNA and a three-plasmid rescue system was established to recover infectious virus. Recombinant virus behaved similarly in cell culture to authentic virus. The ORF for the non-structural NSs protein, encoded on the smallest genome segment, was disrupted by introduction of translation stop codons in the appropriate cDNA, and when this plasmid was used in reverse genetics, a recombinant virus that lacked NSs expression was recovered. This virus had reduced capacity to shut-off host-cell protein synthesis compared with the wild-type virus. In addition, the NSs-deleted virus induced interferon (IFN) in cells, indicating that, like other orthobunyaviruses, NSs functions as an IFN antagonist, most probably by globally inhibiting host-cell metabolism. The development of a robust reverse genetics system for SBV will facilitate investigation of its pathogenic mechanisms as well as the creation of attenuated strains that could be candidate vaccines. | |
dc.format.extent | 9 | |
dc.format.extent | 542872 | |
dc.language.iso | eng | |
dc.relation.ispartof | Journal of General Virology | en |
dc.subject | Valley fever virus | en |
dc.subject | Defective interfering particles | en |
dc.subject | CDNA-based rescue | en |
dc.subject | NSS protein | en |
dc.subject | Untranslated regions | en |
dc.subject | Family bunyaviridae | en |
dc.subject | Replication | en |
dc.subject | RNA | en |
dc.subject | Lacking | en |
dc.subject | Genome | en |
dc.subject | QR355 Virology | en |
dc.subject | SDG 3 - Good Health and Well-being | en |
dc.subject.lcc | QR355 | en |
dc.title | Establishment of a reverse genetics system for Schmallenberg virus, a newly emerged orthobunyavirus in Europe | en |
dc.type | Journal article | en |
dc.contributor.sponsor | The Wellcome Trust | en |
dc.contributor.sponsor | BBSRC | en |
dc.contributor.sponsor | Medical Research Council | en |
dc.contributor.institution | University of St Andrews. School of Biology | en |
dc.contributor.institution | University of St Andrews. Biomedical Sciences Research Complex | en |
dc.identifier.doi | 10.1099/vir.0.049981-0 | |
dc.description.status | Peer reviewed | en |
dc.identifier.grantnumber | 079810/Z/06/Z | en |
dc.identifier.grantnumber | BB/G004277/1 | en |
dc.identifier.grantnumber | G0800161 | en |
This item appears in the following Collection(s)
Items in the St Andrews Research Repository are protected by copyright, with all rights reserved, unless otherwise indicated.