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dc.contributor.authorKillip, M. J.
dc.contributor.authorYoung, D. F.
dc.contributor.authorGatherer, D.
dc.contributor.authorRoss, C. S.
dc.contributor.authorShort, J. A. L.
dc.contributor.authorDavison, A. J.
dc.contributor.authorGoodbourn, S.
dc.contributor.authorRandall, R. E.
dc.identifier.citationKillip , M J , Young , D F , Gatherer , D , Ross , C S , Short , J A L , Davison , A J , Goodbourn , S & Randall , R E 2013 , ' Deep sequencing analysis of defective genomes of parainfluenza virus 5 and their role in interferon induction ' , Journal of Virology , vol. 87 , no. 9 , pp. 4798-4807 .
dc.identifier.otherPURE: 53493229
dc.identifier.otherPURE UUID: 5749c7ab-e7e8-4c4b-b858-8256db1d8c13
dc.identifier.otherWOS: 000317416400002
dc.identifier.otherScopus: 84876318226
dc.identifier.otherORCID: /0000-0002-9304-6678/work/60426992
dc.descriptionThis work was supported by The Wellcome Trust (087751/A/08/Z).en
dc.description.abstractPreparations of parainfluenza virus 5 (PIV5) that are potent activators of the interferon (IFN) induction cascade were generated by high-multiplicity passage in order to accumulate defective interfering virus genomes (DIs). Nucleocapsid RNA from these virus preparations was extracted and subjected to deep sequencing. Sequencing data were analyzed using methods designed to detect internal deletion and "copyback" DIs in order to identify and characterize the different DIs present and to approximately quantify the ratio of defective to nondefective genomes. Trailer copybacks dominated the DI populations in IFN-inducing preparations of both the PIV5 wild type (wt) and PIV5-V Delta C (a recombinant virus that does not encode a functional V protein). Although the PIV5 V protein is an efficient inhibitor of the IFN induction cascade, we show that nondefective PIV5 wt is unable to prevent activation of the IFN response by coinfecting copyback DIs due to the interfering effects of copyback DIs on nondefective virus protein expression. As a result, copyback DIs are able to very rapidly activate the IFN induction cascade prior to the expression of detectable levels of V protein by coinfecting nondefective virus.
dc.relation.ispartofJournal of Virologyen
dc.rightsCopyright © 2013, American Society for Microbiology. This is a paid open access article.en
dc.subjectParamyxovirus V proteinsen
dc.subjectInternal deletionsen
dc.subject5'-triphosphate RNAen
dc.subjectIFN-Beta promoteren
dc.subjectViral-RNA Synthesisen
dc.subjectNegative-strand virusen
dc.subjectAntiviral responsesen
dc.titleDeep sequencing analysis of defective genomes of parainfluenza virus 5 and their role in interferon inductionen
dc.typeJournal articleen
dc.description.versionPublisher PDFen
dc.contributor.institutionUniversity of St Andrews. School of Biologyen
dc.contributor.institutionUniversity of St Andrews. Biomedical Sciences Research Complexen
dc.description.statusPeer revieweden

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