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PELDOR in rotationally symmetric homo-oligomers
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dc.contributor.author | Giannoulis, Angeliki | |
dc.contributor.author | Ward, Richard James | |
dc.contributor.author | Branigan, Emma | |
dc.contributor.author | Naismith, Jim | |
dc.contributor.author | Bode, Bela Ernest | |
dc.date.accessioned | 2013-07-23T15:01:02Z | |
dc.date.available | 2013-07-23T15:01:02Z | |
dc.date.issued | 2013 | |
dc.identifier | 51219112 | |
dc.identifier | e0a97a28-cb7f-4c47-a5e6-2a35827790fd | |
dc.identifier | 000325389800018 | |
dc.identifier | 84885641765 | |
dc.identifier.citation | Giannoulis , A , Ward , R J , Branigan , E , Naismith , J & Bode , B E 2013 , ' PELDOR in rotationally symmetric homo-oligomers ' , Molecular Physics , vol. 111 , no. 18-19 , pp. 2845-2854 . https://doi.org/10.1080/00268976.2013.798697 | en |
dc.identifier.issn | 0026-8976 | |
dc.identifier.other | ORCID: /0000-0002-3384-271X/work/27582734 | |
dc.identifier.uri | https://hdl.handle.net/10023/3865 | |
dc.description | This article was made open access through BIS OA funding. | en |
dc.description.abstract | Nanometre distance measurements by pulsed electron−electron double resonance (PELDOR) spectroscopy have become an increasingly important tool in structural biology. The theoretical underpinning of the experiment is well-defined for systems containing two nitroxide spin-labels (spin pairs) however recently experiments have been reported on homo-oligomeric membrane proteins consisting of up to eight spin-labelled monomers. We have explored the theory behind these systems by examining model systems based on multiple spins arranged in rotationally symmetric polygons. The results demonstrate that with a rising number of spins within the test molecule, increasingly strong distortions appear in distance distributions obtained from an analysis based on the simple spin pair approach. These distortions are significant over a range of system sizes and remain so even when random errors are introduced into the symmetry of the model. We present an alternative approach to the extraction of distances on such systems based on a minimisation that properly treats multi-spin correlations. We demonstrate the utility of this approach on a spin-labelled mutant of the heptameric Mechanosensitive Channel of Small Conductance of E. coli. | |
dc.format.extent | 459119 | |
dc.language.iso | eng | |
dc.relation.ispartof | Molecular Physics | en |
dc.subject | DEER | en |
dc.subject | Pulsed dipolar spectroscopy | en |
dc.subject | Multiple spin effects | en |
dc.subject | Homo-oligomer | en |
dc.subject | EPR | en |
dc.subject | Q Science | en |
dc.subject.lcc | Q | en |
dc.title | PELDOR in rotationally symmetric homo-oligomers | en |
dc.type | Journal article | en |
dc.contributor.sponsor | European Commission | en |
dc.contributor.sponsor | BBSRC | en |
dc.contributor.institution | University of St Andrews. School of Chemistry | en |
dc.contributor.institution | University of St Andrews. Centre of Magnetic Resonance | en |
dc.contributor.institution | University of St Andrews. Biomedical Sciences Research Complex | en |
dc.contributor.institution | University of St Andrews. School of Biology | en |
dc.contributor.institution | University of St Andrews. EaSTCHEM | en |
dc.identifier.doi | https://doi.org/10.1080/00268976.2013.798697 | |
dc.description.status | Peer reviewed | en |
dc.identifier.grantnumber | en | |
dc.identifier.grantnumber | BB/H017917/1 | en |
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