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CRISPR interference : a structural perspective

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Date
06/2013
Author
Reeks, Judith Anne
Naismith, Jim
White, Malcolm F
Funder
BBSRC
BBSRC
Grant ID
BB/K000314/1
BB/G011400/1
Keywords
Antiviral defence
Cluster of regularly interspaced palindromic repeats (CRISPR)
Crystallography
Evolution
Protein structure
Repeat-associated mysterious protein (RAMP)
Q Science
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Abstract
CRISPR (cluster of regularly interspaced palindromic repeats) is a prokaryotic adaptive defence system, providing immunity against mobile genetic elements such as viruses. Genomically encoded crRNA (CRISPR RNA) is used by Cas (CRISPR-associated) proteins to target and subsequently degrade nucleic acids of invading entities in a sequence-dependent manner. The process is known as ‘interference’. In the present review we cover recent progress on the structural biology of the CRISPR/Cas system, focusing on the Cas proteins and complexes that catalyse crRNA biogenesis and interference. Structural studies have helped in the elucidation of key mechanisms, including the recognition and cleavage of crRNA by the Cas6 and Cas5 proteins, where remarkable diversity at the level of both substrate recognition and catalysis has become apparent. The RNA-binding RAMP (repeat-associated mysterious protein) domain is present in the Cas5, Cas6, Cas7 and Cmr3 protein families and RAMP-like domains are found in Cas2 and Cas10. Structural analysis has also revealed an evolutionary link between the small subunits of the type I and type III-B interference complexes. Future studies of the interference complexes and their constituent components will transform our understanding of the system.
Citation
Reeks , J A , Naismith , J & White , M F 2013 , ' CRISPR interference : a structural perspective ' , Biochemical Journal , vol. 453 , no. 2 , pp. 155-166 . https://doi.org/10.1042/BJ20130316
Publication
Biochemical Journal
Status
Peer reviewed
DOI
https://doi.org/10.1042/BJ20130316
ISSN
0264-6021
Type
Journal item
Rights
© 2013 The Authors. The authors have paid for this article to be freely available under the terms of the Creative Commons Attribution Licence (CC-BY)(http://creativecommons.org/licenses/by/3.0/) which permits unrestricted use, distribution and reproduction in any medium, provided the original work is properly cited.
Description
This article was made open access through BIS OA funding. The laboratory is funded by grants from the Biotechnology and Biological Sciences Research Council (BBSRC).
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  • University of St Andrews Research
URI
http://hdl.handle.net/10023/3863

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