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dc.contributor.authorReeks, Judith Anne
dc.contributor.authorGraham, Shirley
dc.contributor.authorAnderson, Linzi
dc.contributor.authorLiu, Huanting
dc.contributor.authorWhite, Malcolm F
dc.contributor.authorNaismith, Jim
dc.date.accessioned2013-05-24T11:01:01Z
dc.date.available2013-05-24T11:01:01Z
dc.date.issued2013-05
dc.identifier.citationReeks , J A , Graham , S , Anderson , L , Liu , H , White , M F & Naismith , J 2013 , ' Structure of the archaeal Cascade subunit Csa5 : Relating the small subunits of CRISPR effector complexes ' RNA Biology , vol. 10 , no. 5 , pp. 762-769 . DOI: 10.4161/rna.23854en
dc.identifier.issn1547-6286
dc.identifier.otherPURE: 53576404
dc.identifier.otherPURE UUID: a41bee5f-dfc3-4cfe-b0c9-27f0f25ac17d
dc.identifier.otherWOS: 000323176900014
dc.identifier.otherScopus: 84879011920
dc.identifier.urihttp://hdl.handle.net/10023/3550
dc.descriptionThis work was funded by a grant from the Biotechnology and Biological Sciences Research Council (BBSRC) (REF: BB/G011400/1) to M.F.W. and J.H.N. and a BBSRC-funded studentship to J.R.en
dc.description.abstractThe Cascade complex for CRISPR-mediated antiviral immunity uses CRISPR RNA (crRNA) to target invading DNA species from mobile elements such as viruses, leading to their destruction. The core of the Cascade effector complex consists of the Cas5 and Cas7 subunits, which are widely conserved in prokaryotes. Cas7 binds crRNA and forms the helical backbone of Cascade. Many archaea encode a version of the Cascade complex (denoted Type I-A) that includes a Csa5 (or small) subunit, which interacts weakly with the core proteins. Here, we report the crystal structure of the Csa5 protein from Sulfolobus solfataricus. Csa5 comprises a conserved α-helical domain with a small insertion consisting of a weakly conserved β-strand domain. In the crystal, the Csa5 monomers have multimerized into infinite helical threads. At each interface is a strictly conserved intersubunit salt bridge, deletion of which disrupts multimerization. Structural analysis indicates a shared evolutionary history among the small subunits of the CRISPR effector complexes. The same α-helical domain is found in the C-terminal domain of Cse2 (from Type I-E Cascade), while the N-terminal domain of Cse2 is found in Cmr5 of the CMR (Type III-B) effector complex. As Cmr5 shares no match with Csa5, two possibilities present themselves: selective domain loss from an ancestral Cse2 to create two new subfamilies or domain fusion of two separate families to create a new Cse2 family. A definitive answer awaits structural studies of further small subunits from other CRISPR effector complexes.en
dc.format.extent8en
dc.language.isoeng
dc.relation.ispartofRNA Biologyen
dc.rights(c) The authors and Landes Bioscience. This is an Open Access article licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported License (http://creativecommons.org/licenses/by-nc/3.0/). The article may redistributed, reproduced and reused for non-commercial purposes, provided the original source is properly cited.en
dc.subjectCRISPRen
dc.subjectCRISPR interferneceen
dc.subjectCascadeen
dc.subjectCsa5en
dc.subjectStructureen
dc.subjectQR355 Virologyen
dc.subject.lccQR355en
dc.titleStructure of the archaeal Cascade subunit Csa5 : Relating the small subunits of CRISPR effector complexesen
dc.typeJournal articleen
dc.description.versionPublisher PDFen
dc.contributor.institutionUniversity of St Andrews. School of Chemistryen
dc.contributor.institutionUniversity of St Andrews. Biomedical Sciences Research Complexen
dc.contributor.institutionUniversity of St Andrews. School of Biologyen
dc.contributor.institutionUniversity of St Andrews. EaSTCHEMen
dc.identifier.doihttps://doi.org/10.4161/rna.23854
dc.description.statusPeer revieweden


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