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dc.contributor.authorRichard, Derek J.
dc.contributor.authorCubeddu, Liza
dc.contributor.authorUrquhart, Aaron J.
dc.contributor.authorBain, Amanda
dc.contributor.authorBolderson, Emma
dc.contributor.authorMenon, Dinoop
dc.contributor.authorWhite, Malcolm F.
dc.contributor.authorKhanna, Kum Kum
dc.date.accessioned2013-03-27T15:31:12Z
dc.date.available2013-03-27T15:31:12Z
dc.date.issued2011-05
dc.identifier.citationRichard , D J , Cubeddu , L , Urquhart , A J , Bain , A , Bolderson , E , Menon , D , White , M F & Khanna , K K 2011 , ' hSSB1 interacts directly with the MRN complex stimulating its recruitment to DNA double-strand breaks and its endo-nuclease activity ' , Nucleic Acids Research , vol. 39 , no. 9 , pp. 3643-3651 . https://doi.org/10.1093/nar/gkq1340en
dc.identifier.issn0305-1048
dc.identifier.otherPURE: 9375546
dc.identifier.otherPURE UUID: db271bb5-1726-4332-8a07-8d41c5ba32c8
dc.identifier.otherWOS: 000290589500020
dc.identifier.otherScopus: 79956004104
dc.identifier.otherORCID: /0000-0003-1543-9342/work/47136133
dc.identifier.urihttps://hdl.handle.net/10023/3439
dc.description.abstracthSSB1 is a recently discovered single-stranded DNA binding protein that is essential for efficient repair of DNA double-strand breaks (DSBs) by the homologous recombination pathway. hSSB1 is required for the efficient recruitment of the MRN complex to sites of DSBs and for the efficient initiation of ATM dependent signalling. Here we explore the interplay between hSSB1 and MRN. We demonstrate that hSSB1 binds directly to NBS1, a component of the MRN complex, in a DNA damage independent manner. Consistent with the direct interaction, we observe that hSSB1 greatly stimulates the endo-nuclease activity of the MRN complex, a process that requires the C-terminal tail of hSSB1. Interestingly, analysis of two point mutations in NBS1, associated with Nijmegen breakage syndrome, revealed weaker binding to hSSB1, suggesting a possible disease mechanism.
dc.format.extent9
dc.language.isoeng
dc.relation.ispartofNucleic Acids Researchen
dc.rights© The Author(s) 2011. Published by Oxford University Press. This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.5/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.en
dc.subjectReplication protein-aen
dc.subjectBinding-proteinen
dc.subjectHomologous recombinationen
dc.subjectMRE11-RAD50-NBS1 COMPLEXen
dc.subjectGenomic stabilityen
dc.subjectS-phaseen
dc.subjectATMen
dc.subjectActivationen
dc.subjectMRE11en
dc.subjectCheckpointen
dc.subjectQH426 Geneticsen
dc.subject.lccQH426en
dc.titlehSSB1 interacts directly with the MRN complex stimulating its recruitment to DNA double-strand breaks and its endo-nuclease activityen
dc.typeJournal articleen
dc.description.versionPublisher PDFen
dc.contributor.institutionUniversity of St Andrews. School of Biologyen
dc.contributor.institutionUniversity of St Andrews. Biomedical Sciences Research Complexen
dc.identifier.doihttps://doi.org/10.1093/nar/gkq1340
dc.description.statusPeer revieweden


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