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dc.contributor.authorReginsson, Gunnar Widtfeldt
dc.contributor.authorShelke, Sandip
dc.contributor.authorRouillon, Christophe
dc.contributor.authorWhite, Malcolm F
dc.contributor.authorSigurdsson, Snorri T
dc.contributor.authorSchiemann, Olav
dc.date.accessioned2012-12-12T10:31:30Z
dc.date.available2012-12-12T10:31:30Z
dc.date.issued2013-01
dc.identifier28835770
dc.identifier0bde46b3-08e9-486e-a927-da04195b2ff9
dc.identifier000312889900011
dc.identifier84871772025
dc.identifier.citationReginsson , G W , Shelke , S , Rouillon , C , White , M F , Sigurdsson , S T & Schiemann , O 2013 , ' Protein-induced changes in DNA structure and dynamics observed with noncovalent site-directed spin-labeling and PELDOR ' , Nucleic Acids Research , vol. 41 , no. 1 , e11 . https://doi.org/10.1093/nar/gks817en
dc.identifier.issn0305-1048
dc.identifier.otherORCID: /0000-0003-1543-9342/work/47136092
dc.identifier.urihttps://hdl.handle.net/10023/3286
dc.description.abstractSite-directed spin labeling and pulsed electron–electron double resonance (PELDOR or DEER) have previously been applied successfully to study the structure and dynamics of nucleic acids. Spin labeling nucleic acids at specific sites requires the covalent attachment of spin labels, which involves rather complicated and laborious chemical synthesis. Here, we use a noncovalent label strategy that bypasses the covalent labeling chemistry and show that the binding specificity and efficiency are large enough to enable PELDOR or DEER measurements in DNA duplexes and a DNA duplex bound to the Lac repressor protein. In addition, the rigidity of the label not only allows resolution of the structure and dynamics of oligonucleotides but also the determination of label orientation and protein-induced conformational changes. The results prove that this labeling strategy in combination with PELDOR has a great potential for studying both structure and dynamics of oligonucleotides and their complexes with various ligands.
dc.format.extent10
dc.format.extent1729247
dc.language.isoeng
dc.relation.ispartofNucleic Acids Researchen
dc.subjectBindingen
dc.subjectPULSED ELECTRONen
dc.subjectNucleic-acidsen
dc.subjectConformationen
dc.subjectLactose operonen
dc.subjectDistance measurementsen
dc.subjectOperatoren
dc.subjectEPR spectroscopyen
dc.subjectElectron-paramagnetic-resonanceen
dc.subjectLAC repressoren
dc.subjectQH426 Geneticsen
dc.subjectQD Chemistryen
dc.subject.lccQH426en
dc.subject.lccQDen
dc.titleProtein-induced changes in DNA structure and dynamics observed with noncovalent site-directed spin-labeling and PELDORen
dc.typeJournal articleen
dc.contributor.sponsorBBSRCen
dc.contributor.sponsorThe Wellcome Trusten
dc.contributor.sponsorBBSRCen
dc.contributor.institutionUniversity of St Andrews. School of Biologyen
dc.contributor.institutionUniversity of St Andrews. Biomedical Sciences Research Complexen
dc.identifier.doi10.1093/nar/gks817
dc.description.statusPeer revieweden
dc.identifier.grantnumberBB/H017917/1en
dc.identifier.grantnumber091825/Z/10/Zen
dc.identifier.grantnumberBB/H017917/1en


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