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dc.contributor.authorReginsson, Gunnar Widtfeldt
dc.contributor.authorShelke, Sandip
dc.contributor.authorRouillon, Christophe
dc.contributor.authorWhite, Malcolm F
dc.contributor.authorSigurdsson, Snorri T
dc.contributor.authorSchiemann, Olav
dc.date.accessioned2012-12-12T10:31:30Z
dc.date.available2012-12-12T10:31:30Z
dc.date.issued2013-01
dc.identifier.citationReginsson , G W , Shelke , S , Rouillon , C , White , M F , Sigurdsson , S T & Schiemann , O 2013 , ' Protein-induced changes in DNA structure and dynamics observed with noncovalent site-directed spin-labeling and PELDOR ' , Nucleic Acids Research , vol. 41 , no. 1 , e11 . https://doi.org/10.1093/nar/gks817en
dc.identifier.issn0305-1048
dc.identifier.otherPURE: 28835770
dc.identifier.otherPURE UUID: 0bde46b3-08e9-486e-a927-da04195b2ff9
dc.identifier.otherWOS: 000312889900011
dc.identifier.otherScopus: 84871772025
dc.identifier.otherORCID: /0000-0003-1543-9342/work/47136092
dc.identifier.urihttp://hdl.handle.net/10023/3286
dc.description.abstractSite-directed spin labeling and pulsed electron–electron double resonance (PELDOR or DEER) have previously been applied successfully to study the structure and dynamics of nucleic acids. Spin labeling nucleic acids at specific sites requires the covalent attachment of spin labels, which involves rather complicated and laborious chemical synthesis. Here, we use a noncovalent label strategy that bypasses the covalent labeling chemistry and show that the binding specificity and efficiency are large enough to enable PELDOR or DEER measurements in DNA duplexes and a DNA duplex bound to the Lac repressor protein. In addition, the rigidity of the label not only allows resolution of the structure and dynamics of oligonucleotides but also the determination of label orientation and protein-induced conformational changes. The results prove that this labeling strategy in combination with PELDOR has a great potential for studying both structure and dynamics of oligonucleotides and their complexes with various ligands.
dc.format.extent10
dc.language.isoeng
dc.relation.ispartofNucleic Acids Researchen
dc.rights© The Author(s) 2012. Published by Oxford University Press. This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.en
dc.subjectBindingen
dc.subjectPULSED ELECTRONen
dc.subjectNucleic-acidsen
dc.subjectConformationen
dc.subjectLactose operonen
dc.subjectDistance measurementsen
dc.subjectOperatoren
dc.subjectEPR spectroscopyen
dc.subjectElectron-paramagnetic-resonanceen
dc.subjectLAC repressoren
dc.subjectQH426 Geneticsen
dc.subjectQD Chemistryen
dc.subject.lccQH426en
dc.subject.lccQDen
dc.titleProtein-induced changes in DNA structure and dynamics observed with noncovalent site-directed spin-labeling and PELDORen
dc.typeJournal articleen
dc.description.versionPublisher PDFen
dc.contributor.institutionUniversity of St Andrews.School of Biologyen
dc.contributor.institutionUniversity of St Andrews.Biomedical Sciences Research Complexen
dc.identifier.doihttps://doi.org/10.1093/nar/gks817
dc.description.statusPeer revieweden


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