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The CRISPR associated protein Cas4 Is a 5' to 3' DNA exonuclease with an iron-sulfur cluster
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dc.contributor.author | Zhang, Jing | |
dc.contributor.author | Kasciukovic, Taciana | |
dc.contributor.author | White, Malcolm F. | |
dc.date.accessioned | 2012-11-27T14:56:36Z | |
dc.date.available | 2012-11-27T14:56:36Z | |
dc.date.issued | 2012-10-08 | |
dc.identifier.citation | Zhang , J , Kasciukovic , T & White , M F 2012 , ' The CRISPR associated protein Cas4 Is a 5' to 3' DNA exonuclease with an iron-sulfur cluster ' , PLoS One , vol. 7 , no. 10 , e47232 . https://doi.org/10.1371/journal.pone.0047232 | en |
dc.identifier.issn | 1932-6203 | |
dc.identifier.other | PURE: 37622656 | |
dc.identifier.other | PURE UUID: da8b62e3-c673-458b-8652-a9e95a82886b | |
dc.identifier.other | WOS: 000309831500148 | |
dc.identifier.other | Scopus: 84867325709 | |
dc.identifier.other | ORCID: /0000-0003-1543-9342/work/47136131 | |
dc.identifier.uri | https://hdl.handle.net/10023/3270 | |
dc.description.abstract | The Cas4 protein is one of the core CRISPR-associated (Cas) proteins implicated in the prokaryotic CRISPR system for antiviral defence. Cas4 is thought to play a role in the capture of new viral DNA sequences for incorporation into the host genome. No biochemical activity has been reported for Cas4, but it is predicted to include a RecB nuclease domain. We show here that Cas4 family proteins from the archaeon Sulfolobus solfataricus utilise four conserved cysteine residues to bind an iron-sulfur cluster in an arrangement reminiscent of the AddB nuclease of Bacillus subtilis. The Cas4 family protein Sso0001 is a 5' to 3' single stranded DNA exonuclease in vitro that is stalled by extrahelical DNA adducts. A role for Cas4 in DNA duplex strand resectioning to generate recombinogenic 3' single stranded DNA overhangs is proposed. Comparison of the AddB structure with that of a related bacterial nuclease from Eubacterium rectales reveals that the iron-sulfur cluster can be replaced by a zinc ion without disrupting the protein structure, with implications for the evolution of iron-sulfur binding proteins. | |
dc.format.extent | 8 | |
dc.language.iso | eng | |
dc.relation.ispartof | PLoS One | en |
dc.rights | © Zhang et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. | en |
dc.subject | QR355 Virology | en |
dc.subject.lcc | QR355 | en |
dc.title | The CRISPR associated protein Cas4 Is a 5' to 3' DNA exonuclease with an iron-sulfur cluster | en |
dc.type | Journal article | en |
dc.contributor.sponsor | BBSRC | en |
dc.contributor.sponsor | BBSRC | en |
dc.description.version | Publisher PDF | en |
dc.contributor.institution | University of St Andrews. School of Biology | en |
dc.contributor.institution | University of St Andrews. Biomedical Sciences Research Complex | en |
dc.identifier.doi | https://doi.org/10.1371/journal.pone.0047232 | |
dc.description.status | Peer reviewed | en |
dc.identifier.grantnumber | BB/G011400/1 | en |
dc.identifier.grantnumber | BB/K000314/1 | en |
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