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RNA processing by the CRISPR-associated NYN ribonuclease
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dc.contributor.author | Chi, Haotian | |
dc.contributor.author | White, Malcolm | |
dc.date.accessioned | 2024-07-09T16:30:09Z | |
dc.date.available | 2024-07-09T16:30:09Z | |
dc.date.issued | 2024-06-17 | |
dc.identifier | 302347485 | |
dc.identifier | 0a81dfd2-61bb-4632-a96a-db0a7a06e43f | |
dc.identifier.citation | Chi , H & White , M 2024 , ' RNA processing by the CRISPR-associated NYN ribonuclease ' , Biochemical Journal , vol. 481 , no. 12 , pp. 793–804 . https://doi.org/10.1042/BCJ20240151 | en |
dc.identifier.issn | 0264-6021 | |
dc.identifier.other | ORCID: /0000-0003-1543-9342/work/163571331 | |
dc.identifier.uri | https://hdl.handle.net/10023/30131 | |
dc.description | Funding: EC | European Research Council (ERC) - 101018608; China Scholarship Council (CSC) - 202008420207. | en |
dc.description.abstract | CRISPR-Cas systems confer adaptive immunity in prokaryotes, facilitating the recognition and destruction of invasive nucleic acids. Type III CRISPR systems comprise large, multisubunit ribonucleoprotein complexes with a catalytic Cas10 subunit. When activated by the detection of foreign RNA, Cas10 generates nucleotide signalling molecules that elicit an immune response by activating ancillary effector proteins. Among these systems, the Bacteroides fragilis type III CRISPR system was recently shown to produce a novel signal molecule, SAM-AMP, by conjugating ATP and SAM. SAM-AMP regulates a membrane effector of the CorA family to provide immunity. Here, we focus on NYN, a ribonuclease encoded within this system, probing its potential involvement in crRNA maturation. Structural modelling and in vitro ribonuclease assays reveal that NYN displays robust sequence-nonspecific, Mn2+-dependent ssRNA-cleavage activity. Our findings suggest a role for NYN in trimming crRNA intermediates into mature crRNAs, which is necessary for type III CRISPR antiviral defence. This study sheds light on the functional relevance of CRISPR-associated NYN proteins and highlights the complexity of CRISPR-mediated defence strategies in bacteria. | |
dc.format.extent | 4394996 | |
dc.language.iso | eng | |
dc.relation.ispartof | Biochemical Journal | en |
dc.subject | Bacteriophages | en |
dc.subject | CRISPR | en |
dc.subject | Endonucleases | en |
dc.subject | Immunology | en |
dc.subject | Non-coding RNA | en |
dc.subject | RR-NDAS | en |
dc.title | RNA processing by the CRISPR-associated NYN ribonuclease | en |
dc.type | Journal article | en |
dc.contributor.sponsor | European Research Council | en |
dc.contributor.institution | University of St Andrews. St Andrews Bioinformatics Unit | en |
dc.contributor.institution | University of St Andrews. Biomedical Sciences Research Complex | en |
dc.contributor.institution | University of St Andrews. School of Biology | en |
dc.identifier.doi | https://doi.org/10.1042/BCJ20240151 | |
dc.description.status | Peer reviewed | en |
dc.identifier.grantnumber | 01018608 | en |
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