Characterisation and mapping of a Globodera pallida resistance derived from the wild potato species Solanum spegazzinii
Abstract
The potato cyst nematodes (PCN) Globodera pallida and Globodera rostochiensis are economically important potato pests in almost all regions where potato is grown. One important management strategy involves deployment through introgression breeding into modern cultivars of new sources of naturally occurring resistance from wild potato species. We describe a new source of resistance to G. pallida from wild potato germplasm. The diploid species Solanum spegazzinii Bitter accession CPC 7195 shows resistance to G. pallida pathotypes Pa1 and Pa2/3. A cross and first backcross of S. spegazzinii with Solanum tuberosum Group Phureja cultivar Mayan Gold were performed, and the level of resistance to G. pallida Pa2/3 was determined in progeny clones. Bulk-segregant analysis (BSA) using generic mapping enrichment sequencing (GenSeq) and genotyping-by-sequencing were performed to identify single-nucleotide polymorphisms (SNPs) that are genetically linked to the resistance, using S. tuberosum Group Phureja clone DM1-3 516 R44 as a reference genome. These SNPs were converted into allele-specific PCR assays, and the resistance was mapped to an interval of roughly 118 kb on chromosome VI. This newly identified resistance, which we call Gpa VIlspg, can be used in future efforts to produce modern cultivars with enhanced and broad-spectrum resistances to the major pests and pathogens of potato.
Citation
Gartner , U , Armstrong , M R , Sharma , S K , Jones , J T , Blok , V C , Hein , I & Bryan , G J 2024 , ' Characterisation and mapping of a Globodera pallida resistance derived from the wild potato species Solanum spegazzinii ' , Theoretical and Applied Genetics , vol. 137 , no. 5 . https://doi.org/10.1007/s00122-024-04605-0
Publication
Theoretical and Applied Genetics
Status
Peer reviewed
ISSN
1432-2242Type
Journal article
Description
This work was funded by the Globodera Alliance project (GLOBAL) (funded by USDA NIFA (Grant No. 2015-69004-23634)) and supported by the Rural and Environment Science and Analytical Services, (RESAS) Division of the Scottish Government through project JHI-B1-1 and the Biotechnology and Biological Sciences Research Council (BBSRC) through awards BB/S015663/1 and BB/X009068/1.Collections
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