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CRISPR antiphage defence mediated by the cyclic nucleotide-binding membrane protein Csx23
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dc.contributor.author | Grüschow, Sabine | |
dc.contributor.author | McQuarrie, Stuart | |
dc.contributor.author | Ackermann, Katrin | |
dc.contributor.author | McMahon, Stephen | |
dc.contributor.author | Bode, Bela E | |
dc.contributor.author | Gloster, Tracey M | |
dc.contributor.author | White, Malcolm F | |
dc.date.accessioned | 2024-03-15T12:30:13Z | |
dc.date.available | 2024-03-15T12:30:13Z | |
dc.date.issued | 2024-04-12 | |
dc.identifier | 300250146 | |
dc.identifier | b54444d3-2eef-4e70-8940-57d3ff5c8c7b | |
dc.identifier | 85190494090 | |
dc.identifier | 38471818 | |
dc.identifier.citation | Grüschow , S , McQuarrie , S , Ackermann , K , McMahon , S , Bode , BE , Gloster , TM & White , MF 2024 , ' CRISPR antiphage defence mediated by the cyclic nucleotide-binding membrane protein Csx23 ' , Nucleic Acids Research , vol. 52 , no. 6 , gkae167 , pp. 2761-2775 . https://doi.org/10.1093/nar/gkae167 | en |
dc.identifier.issn | 0305-1048 | |
dc.identifier.other | RIS: urn:4FED0C00067E7BC8F42D7184FCF23318 | |
dc.identifier.other | ORCID: /0000-0002-3384-271X/work/155626529 | |
dc.identifier.other | ORCID: /0000-0003-4828-4842/work/155627282 | |
dc.identifier.other | ORCID: /0000-0003-1543-9342/work/155627316 | |
dc.identifier.uri | https://hdl.handle.net/10023/29505 | |
dc.description | Funding: Biotechnology and Biological Sciences Research Council [BB/T004789/1 to M.F.W., T.M.G.]; European Research Council Advanced Grant [101018608 to M.F.W.]; Engineering and Physical Sciences Research Council [EP/X016455/1 to K.A., B.E.B., M.F.W.]; BBSRC equipment grants [BB/R013780/1, BB/T017740/1 to B.E.B.]. Funding for open access charge: University of St Andrews block grant. | en |
dc.description.abstract | CRISPR-Cas provides adaptive immunity in prokaryotes. Type III CRISPR systems detect invading RNA and activate the catalytic Cas10 subunit, which generates a range of nucleotide second messengers to signal infection. These molecules bind and activate a diverse range of effector proteins that provide immunity by degrading viral components and/or by disturbing key aspects of cellular metabolism to slow down viral replication. Here, we focus on the uncharacterised effector Csx23, which is widespread in Vibrio cholerae. Csx23 provides immunity against plasmids and phage when expressed in Escherichia coli along with its cognate type III CRISPR system. The Csx23 protein localises in the membrane using an N-terminal transmembrane α-helical domain and has a cytoplasmic C-terminal domain that binds cyclic tetra-adenylate (cA4), activating its defence function. Structural studies reveal a tetrameric structure with a novel fold that binds cA4 specifically. Using pulse EPR, we demonstrate that cA4 binding to the cytoplasmic domain of Csx23 results in a major perturbation of the transmembrane domain, consistent with the opening of a pore and/or disruption of membrane integrity. This work reveals a new class of cyclic nucleotide binding protein and provides key mechanistic detail on a membrane-associated CRISPR effector. | |
dc.format.extent | 15 | |
dc.format.extent | 3111357 | |
dc.language.iso | eng | |
dc.relation.ispartof | Nucleic Acids Research | en |
dc.subject | Genetics | en |
dc.subject | DAS | en |
dc.subject | MCC | en |
dc.title | CRISPR antiphage defence mediated by the cyclic nucleotide-binding membrane protein Csx23 | en |
dc.type | Journal article | en |
dc.contributor.sponsor | BBSRC | en |
dc.contributor.sponsor | EPSRC | en |
dc.contributor.sponsor | BBSRC | en |
dc.contributor.sponsor | BBSRC | en |
dc.contributor.sponsor | European Research Council | en |
dc.contributor.institution | University of St Andrews. School of Biology | en |
dc.contributor.institution | University of St Andrews. Biomedical Sciences Research Complex | en |
dc.contributor.institution | University of St Andrews. School of Chemistry | en |
dc.contributor.institution | University of St Andrews. Institute of Behavioural and Neural Sciences | en |
dc.contributor.institution | University of St Andrews. EaSTCHEM | en |
dc.contributor.institution | University of St Andrews. Centre of Magnetic Resonance | en |
dc.contributor.institution | University of St Andrews. St Andrews Bioinformatics Unit | en |
dc.identifier.doi | 10.1093/nar/gkae167 | |
dc.description.status | Peer reviewed | en |
dc.identifier.grantnumber | BB/T004789/1 | en |
dc.identifier.grantnumber | EP/X016455/1 | en |
dc.identifier.grantnumber | BB/R013780/1 | en |
dc.identifier.grantnumber | BB/T017740/1 | en |
dc.identifier.grantnumber | 01018608 | en |
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