Evaluation of dual application of Photodynamic Therapy - PDT in Candida albicans
Abstract
This study aimed to evaluate, in vitro, the efficacy of photodynamic therapy - PDT using dimethyl methylene blue zinc chloride double salt (DMMB) and red LED light on planktonic cultures of Candida albicans. The tests were performed using the ATCC 90028 strain grown at 37°C for 24-h, according to a growth curve of C. albicans. The colonies were resuspended in sterile saline adjusted to a concentration of 2 × 108 cells / mL, with three experimental protocols being tested (Protocol 1, 2 and 3) with a fixed concentration of 750 ɳg/mL obtained through the IC50, and energy density 20 J/cm2. Protocol 1 was carried out using conventional PDT, Protocol 2 was applied double PDT in a single session, and Protocol 3 was applied double PDT in two sessions with a 24-h interval. The results showed logarithmic reductions of 3 (4.252575 ± 0.068526) and 4 logs (2.669533 ± 0.058592) of total fungal load in protocols 3 and 2 respectively in comparison to the Control (6.633547 ± 0.065384). Our results indicated that double application in a single session of PDT was the most effective approach for inhibiting the proliferation of Candida albicans (99.991 % inhibition).
Citation
Nunes , I P F , Crugeira , P J L , Sampaio , F J P , de Oliveira , S C P S , Azevedo , J M , Santos , C L O , Soares , L G P , Samuel , I D W , Persheyev , S , de Ameida , P F & Pinheiro , A L B 2023 , ' Evaluation of dual application of Photodynamic Therapy - PDT in Candida albicans ' , Photodiagnosis and Photodynamic Therapy , vol. 42 , 103327 . https://doi.org/10.1016/j.pdpdt.2023.103327
Publication
Photodiagnosis and Photodynamic Therapy
Status
Peer reviewed
ISSN
1572-1000Type
Journal article
Description
Funding: Grants from the Brazilian National Council for Scientific and Technological Development (CNPq) (No.304279/2018-8, No. 302753/2020-6 and Projeto PIBIT-UFBA n° 16476); Grants from the Bahia State Research Support Foundation) FAPESB (BOL0777/2016 and BOL0316/2018), and the British Council (SPA0-XGB-008).Collections
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