Snapshot hyperspectral imaging of intracellular lasers
Abstract
Intracellular lasers are emerging as powerful biosensors for multiplexed tracking and precision sensing of cells and their microenvironment. This sensing capacity is enabled by quantifying their narrow-linewidth emission spectra, which is presently challenging to do at high speeds. In this work, we demonstrate rapid snapshot hyperspectral imaging of intracellular lasers. Using integral field mapping with a microlens array and a diffraction grating, we obtain images of the spatial and spectral intensity distribution from a single camera acquisition. We demonstrate widefield hyperspectral imaging over a 3 × 3 mm2 field of view and volumetric imaging over 250 × 250 × 800 µm3 (XYZ) volumes with a lateral (XY) resolution of 5 µm, axial (Z) resolution of 10 µm, and a spectral resolution of less than 0.8 nm. We evaluate the performance and outline the challenges and strengths of snapshot methods in the context of characterizing the emission from intracellular lasers. This method offers new opportunities for a diverse range of applications, including high-throughput and long-term biosensing with intracellular lasers.
Citation
Caixeiro , S , Wijesinghe , P , Dholakia , K & Gather , M C 2023 , ' Snapshot hyperspectral imaging of intracellular lasers ' , Optics Express , vol. 31 , no. 20 . https://doi.org/10.1364/OE.498022
Publication
Optics Express
Status
Peer reviewed
ISSN
1094-4087Type
Journal article
Description
This work received financial support from a UK EPSRC Programme Grant (EP/P030017/1). PW was supported by the 1851 Research Fellowship from the Royal Commission. KD acknowledges support from the Australian Research Council (FL210100099). MCG acknowledges support from the Alexander von Humboldt Foundation (Humboldt professorship).Collections
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