Transcription factor NFE2L1 decreases in glomerulonephropathies after podocyte damage
Abstract
Podocyte cellular injury and detachment from glomerular capillaries constitute a critical factor contributing to kidney disease. Notably, transcription factors are instrumental in maintaining podocyte differentiation and homeostasis. This study explores the hitherto uninvestigated expression of Nuclear Factor Erythroid 2-related Factor 1 (NFE2L1) in podocytes. We evaluated the podocyte expression of NFE2L1, Nuclear Factor Erythroid 2-related Factor 2 (NFE2L2), and NAD(P)H:quinone Oxidoreductase (NQO1) in 127 human glomerular disease biopsies using multiplexed immunofluorescence and image analysis. We found that both NFE2L1 and NQO1 expressions were significantly diminished across all observed renal diseases. Furthermore, we exposed human immortalized podocytes and ex vivo kidney slices to Puromycin Aminonucleoside (PAN) and characterized the NFE2L1 protein isoform expression. PAN treatment led to a reduction in the nuclear expression of NFE2L1 in ex vivo kidney slices and podocytes.
Citation
Elshani , M , Um , I H , Leung , S , Reynolds , P A , Chapman , A , Kudsy , M & Harrison , D J 2023 , ' Transcription factor NFE2L1 decreases in glomerulonephropathies after podocyte damage ' , Cells , vol. 12 , no. 17 , 2165 . https://doi.org/10.3390/cells12172165
Publication
Cells
Status
Peer reviewed
ISSN
2073-4409Type
Journal article
Description
Funding: This work was supported by NHS Lothian. This project received funding from the European Union’s Horizon 2020 research and innovation programme under Grant Agreement No. 101017453 as part of the KATY project, and from NuCana plc.Collections
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