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dc.contributor.authorShah, Anokhi
dc.contributor.authorTaylor, Michael J
dc.contributor.authorMolinaro, Giulia
dc.contributor.authorAnbu, Sellamuthu
dc.contributor.authorVerdu, Margaux
dc.contributor.authorJennings, Lucy
dc.contributor.authorMikulska, Iuliia
dc.contributor.authorDiaz-Moreno, Sofia
dc.contributor.authorEl Mkami, Hassane
dc.contributor.authorSmith, Graham M
dc.contributor.authorBritton, Melanie M
dc.contributor.authorLovett, Janet E
dc.contributor.authorPeacock, Anna F A
dc.identifier.citationShah , A , Taylor , M J , Molinaro , G , Anbu , S , Verdu , M , Jennings , L , Mikulska , I , Diaz-Moreno , S , El Mkami , H , Smith , G M , Britton , M M , Lovett , J E & Peacock , A F A 2023 , ' Design of the elusive proteinaceous oxygen donor copper site suggests a promising future for copper for MRI contrast agents ' , Proceedings of the National Academy of Sciences of the United States of America , vol. 120 , no. 27 , e2219036120 .
dc.identifier.otherJisc: 1197308
dc.identifier.otherpmc: PMC10318980
dc.identifier.otherORCID: /0000-0002-0552-5784/work/139551751
dc.identifier.otherORCID: /0000-0001-6578-7412/work/139554022
dc.identifier.otherORCID: /0000-0002-3561-450X/work/139554674
dc.descriptionWe thank the Centre for Chemical and Materials Analysis in the School of Chemistry for support of this research. We thank Diamond Light Source for access to beamline I20-Scanning (proposal number SP18941), Horizon 2020 for a Marie Skłodowska-Curie Individual Fellowship (H2020-MSCA-IF-2014-658843) for S.A., EPSRC for funding (EP/K039245/1, EP/R511651/1). We thank the Wellcome Trust for multiuser equipment grant (099149/Z/12/Z) for the high-power Q-band EPR spectrometer and BBSRC (BB/T017740/1) including for the cryogen-free cooling upgrade to the existing X-band (BB/R013780/1). J.E.L. thanks the Royal Society for a University Research Fellowship. M.J.T. and A.S. thank EPSRC for doctoral funding (EP/M508214/1) and (EP/L015110/1), respectively.en
dc.description.abstractWe report the preparation and spectroscopic characterization of a highly elusive copper site bound exclusively to oxygen donor atoms within a protein scaffold. Despite copper generally being considered unsuitable for use in MRI contrast agents, which in the clinic are largely Gd(III) based, the designed copper coiled coil displays relaxivity values equal to, or superior than, those of the Gd(III) analog at clinical field strengths. The creation of this new-to-biology proteinaceous CuO -binding site demonstrates the power of the de novo peptide design approach to access chemistry for abiological applications, such as for the development of MRI contrast agents.
dc.relation.ispartofProceedings of the National Academy of Sciences of the United States of Americaen
dc.subjectcoiled coilen
dc.subjectMagnetic Resonance Imagingen
dc.subjectBinding Sitesen
dc.subjectbioinorganic chemistryen
dc.subjectcontrast agentsen
dc.subjectContrast Media - chemistryen
dc.subjectprotein designen
dc.subjectCopper - metabolismen
dc.titleDesign of the elusive proteinaceous oxygen donor copper site suggests a promising future for copper for MRI contrast agentsen
dc.typeJournal articleen
dc.contributor.sponsorThe Wellcome Trusten
dc.contributor.sponsorThe Royal Societyen
dc.contributor.institutionUniversity of St Andrews. Biomedical Sciences Research Complexen
dc.contributor.institutionUniversity of St Andrews. School of Physics and Astronomyen
dc.description.statusPeer revieweden

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