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dc.contributor.authorWoodford, Luke
dc.contributor.authorSteketee, Pieter C
dc.contributor.authorEvans, David John
dc.date.accessioned2023-06-28T12:30:10Z
dc.date.available2023-06-28T12:30:10Z
dc.date.issued2023-06-28
dc.identifier286587996
dc.identifier76298ce0-8a50-4574-8bf7-47ebee169925
dc.identifier85163533310
dc.identifier.citationWoodford , L , Steketee , P C & Evans , D J 2023 , ' Doomed drones? Using passage experiments and mathematical modelling to determine Deformed wing virus population dynamics in male honey bees ' , Proceedings of the Royal Society B: Biological Sciences , vol. 290 , no. 2001 , 20231010 . https://doi.org/10.1098/rspb.2023.1010en
dc.identifier.issn1471-2954
dc.identifier.otherORCID: /0000-0002-1315-4258/work/137914987
dc.identifier.urihttps://hdl.handle.net/10023/27819
dc.descriptionFunding: This research was funded by the BBSRC (Biotechnology and Biological Sciences Research Council), grant numbers: BB/M010996/1 and BB/R00305X/1. P.C.S was supported by the BBSRC, grant number: BB/S00243X/1.en
dc.description.abstractVarroa destructor is an ectoparasitic mite of honeybees which vectors a range of pathogenic viruses, the most notable being Deformed wing virus (DWV). Mites parasitise bees during pupal development and male honeybees, drones, have a longer development cycle than female workers (24 versus 21 days), allow for more progeny mites to develop per foundress (1.6–2.5 compared to 0.7–1.45). How this longer exposure time influences evolution of the transmitted virus population is unknown. Using uniquely tagged viruses recovered from cDNA we investigated the replication, competition and morbidity of DWV genotypes in drones. Assays examining virus replication and morbidity revealed drones are highly susceptible to both predominant genotypes of DWV. In virus passage studies using an equimolar inocula of major DNA genotypes and their recombinants, the recombinant form dominated but did not reach 100% of the virus population within 10 passages. Using an in-silico model of the virus–mite–bee system we examined bottlenecks during virus acquisition by the mite and subsequent injection of viruses into the host, which may play a significant role in shaping virus diversity. This study furthers our understanding of the variables influencing DWV diversity changes and provides insight into areas of future research in the mite–virus–bee system.
dc.format.extent11
dc.format.extent1559107
dc.language.isoeng
dc.relation.ispartofProceedings of the Royal Society B: Biological Sciencesen
dc.subjectVarroaen
dc.subjectDroneen
dc.subjectMathematical modelen
dc.subjectHoneybeeen
dc.subjectDWVen
dc.subjectQR355 Virologyen
dc.subjectDASen
dc.subjectMCCen
dc.subject.lccQR355en
dc.titleDoomed drones? Using passage experiments and mathematical modelling to determine Deformed wing virus population dynamics in male honey beesen
dc.typeJournal articleen
dc.contributor.sponsorBBSRCen
dc.contributor.institutionUniversity of St Andrews. School of Biologyen
dc.identifier.doi10.1098/rspb.2023.1010
dc.description.statusPeer revieweden
dc.identifier.grantnumberBB/R00305X/1en


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