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dc.contributor.authorCaixeiro , Soraya
dc.contributor.authorWijesinghe, Philip
dc.contributor.authorDholakia, Kishan
dc.contributor.authorGather, Malte Christian
dc.identifier.citationCaixeiro , S , Wijesinghe , P , Dholakia , K & Gather , M C 2023 ' Snapshot hyperspectral imaging of intracellular lasers ' arXiv .
dc.identifier.otherPURE: 287058910
dc.identifier.otherPURE UUID: 4878717c-d288-4211-b445-679d1474aea9
dc.descriptionThis work received financial support from a UK EPSRC Programme Grant (EP/P030017/1). PW was supported by the 1851 Research Fellowship from the Royal Commission. KD acknowledges support from the Australian Research Council (FL210100099). MCG acknowledges support from the Alexander von Humboldt Foundation (Humboldt professorship).en
dc.description.abstractIntracellular lasers are emerging as powerful biosensors for multiplexed tracking and precision sensing of cells and their microenvironment. This sensing capacity is enabled by quantifying their narrow-linewidth emission spectra, which is presently challenging to do at high speeds. In this work, we demonstrate rapid snapshot hyperspectral imaging of intracellular lasers. Using integral field mapping with a microlens array and a diffraction grating, we obtain images of the spatial and spectral intensity distribution from a single camera acquisition. We demonstrate widefield hyperspectral imaging over a 3 × 3 mm2 field of view and volumetric imaging over 250 × 250 × 800 µm3 (XYZ) volumes with a lateral (XY) resolution of 5 µm, axial (Z) resolution of 10 µm, and a spectral resolution of less than 0.8 nm. We evaluate the performance and outline the challenges and strengths of snapshot methods in the context of characterizing the emission from intracellular lasers. This method offers new opportunities for a diverse range of applications, including high-throughput and long-term biosensing with intracellular lasers.
dc.rightsCopyright © 2023 the Author(s).en
dc.subjectWhispering gallery mode lasersen
dc.subjectIntracellular lasingen
dc.subjectLaser particlesen
dc.subjectIntegral field mappingen
dc.titleSnapshot hyperspectral imaging of intracellular lasersen
dc.typeWorking or discussion paperen
dc.contributor.institutionUniversity of St Andrews. School of Physics and Astronomyen
dc.contributor.institutionUniversity of St Andrews. Centre for Biophotonicsen

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