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dc.contributor.authorFisher, Gemma
dc.contributor.authorPecaver, Ennio
dc.contributor.authorRead, Benjamin
dc.contributor.authorLeese, Susannah
dc.contributor.authorLaing, Erin
dc.contributor.authorDickson, Alison
dc.contributor.authorMelo Czekster, Clarissa
dc.contributor.authorda Silva, R.G.
dc.date.accessioned2023-05-26T10:30:11Z
dc.date.available2023-05-26T10:30:11Z
dc.date.issued2023-06-02
dc.identifier.citationFisher , G , Pecaver , E , Read , B , Leese , S , Laing , E , Dickson , A , Melo Czekster , C & da Silva , R G 2023 , ' Catalytic cycle of the bifunctional enzyme phosphoribosyl-ATP pyrophosphohydrolase/phosphoribosyl-AMP cyclohydrolase ' , ACS Catalysis , vol. 13 , no. 11 , pp. 7669-7679 . https://doi.org/10.1021/acscatal.3c01111en
dc.identifier.issn2155-5435
dc.identifier.otherPURE: 285807858
dc.identifier.otherPURE UUID: 7b98e5aa-f322-4ba1-a72d-3673f683186f
dc.identifier.otherORCID: /0000-0002-7163-4057/work/135850722
dc.identifier.otherORCID: /0000-0002-4150-2467/work/135851104
dc.identifier.otherORCID: /0000-0002-1308-8190/work/135851284
dc.identifier.otherScopus: 85162830660
dc.identifier.urihttps://hdl.handle.net/10023/27680
dc.descriptionFunding: This study was supported by the Biotechnology and Biological Sciences Research Council (BBSRC) (Grant BB/M010996/1) via EASTBIO Doctoral Training Partnership studentships to G.F. and B.J.R., and by the University of St Andrews via a StARIS summer research bursary to S.K.L.en
dc.description.abstractThe bifunctional enzyme phosphoribosyl-ATP pyrophosphohydrolase/phosphoribosyl-AMP cyclohydrolase (HisIE) catalyzes the second and third steps of histidine biosynthesis: pyrophosphohydrolysis of N1-(5-phospho-β-D-ribosyl)-ATP (PRATP) to N1-(5-phospho-β-D-ribosyl)-AMP (PRAMP) and pyrophosphate in the C-terminal HisE-like domain, and cyclohydrolysis of PRAMP to N-(5′-phospho-D-ribosylformimino)-5-amino-1-(5″-phospho-D-ribosyl)-4-imidazolecarboxamide (ProFAR) in the N-terminal HisI-like domain. Here we use UV–VIS spectroscopy and LC–MS to show Acinetobacter baumannii putative HisIE produces ProFAR from PRATP. Employing an assay to detect pyrophosphate and another to detect ProFAR, we established the pyrophosphohydrolase reaction rate is higher than the overall reaction rate. We produced a truncated version of the enzyme-containing only the C-terminal (HisE) domain. This truncated HisIE was catalytically active, which allowed the synthesis of PRAMP, the substrate for the cyclohydrolysis reaction. PRAMP was kinetically competent for HisIE-catalyzed ProFAR production, demonstrating PRAMP can bind the HisI-like domain from bulk water, and suggesting that the cyclohydrolase reaction is rate-limiting for the overall bifunctional enzyme. The overall kcat increased with increasing pH, while the solvent deuterium kinetic isotope effect decreased at more basic pH but was still large at pH 7.5. The lack of solvent viscosity effects on kcat and kcat/KM ruled out diffusional steps limiting the rates of substrate binding and product release. Rapid kinetics with excess PRATP demonstrated a lag time followed by a burst in ProFAR formation. These observations are consistent with a rate-limiting unimolecular step involving a proton transfer following adenine ring opening. We synthesized N1-(5-phospho-β-D-ribosyl)-ADP (PRADP), which could not be processed by HisIE. PRADP inhibited HisIE-catalyzed ProFAR formation from PRATP but not from PRAMP, suggesting that it binds to the phosphohydrolase active site while still permitting unobstructed access of PRAMP to the cyclohydrolase active site. The kinetics data are incompatible with a build-up of PRAMP in bulk solvent, indicating HisIE catalysis involves preferential channeling of PRAMP, albeit not via a protein tunnel.
dc.format.extent11
dc.language.isoeng
dc.relation.ispartofACS Catalysisen
dc.rightsCopyright © 2023 The Authors. Published by American Chemical Society under a CC BY license.en
dc.subjectPhosphoribosyl-ATP pyrophosphohydrolaseen
dc.subjectPhosphoribosyl-AMP cyclohydrolaseen
dc.subjectAcinetobacter baumanniien
dc.subjectEnzyme kineticsen
dc.subjectHistidine biosynthesisen
dc.subjectKinetic isotope effectsen
dc.subjectSubstrate channelingen
dc.subjectQD Chemistryen
dc.subjectNDASen
dc.subjectMCCen
dc.subject.lccQDen
dc.titleCatalytic cycle of the bifunctional enzyme phosphoribosyl-ATP pyrophosphohydrolase/phosphoribosyl-AMP cyclohydrolaseen
dc.typeJournal articleen
dc.description.versionPublisher PDFen
dc.contributor.institutionUniversity of St Andrews. School of Biologyen
dc.contributor.institutionUniversity of St Andrews. School of Medicineen
dc.contributor.institutionUniversity of St Andrews. Biomedical Sciences Research Complexen
dc.identifier.doihttps://doi.org/10.1021/acscatal.3c01111
dc.description.statusPeer revieweden


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