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Third harmonic imaging contrast from tubular structures in the presence of index discontinuity

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Morizet_2023_SR_Third_harmonic_CC.pdf (4.504Mb)
Date
15/05/2023
Author
Morizet, Joséphine
Olivier, Nicolas
Mahou, Pierre
Boutillon, Arthur
Stringari, Chiara
Beaurepaire, Emmanuel
Keywords
3rd-DAS
MCP
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Abstract
Accurate interpretation of third harmonic generation (THG) microscopy images in terms of sample optical properties and microstructure is generally hampered by the presence of excitation field distortions resulting from sample heterogeneity. Numerical methods that account for these artifacts need to be established. In this work, we experimentally and numerically analyze the THG contrast obtained from stretched hollow glass pipettes embedded in different liquids. We also characterize the nonlinear optical properties of 2,2′-thiodiethanol (TDE), a water-soluble index-matching medium. We find that index discontinuity not only changes the level and modulation amplitude of polarization-resolved THG signals, but can even change the polarization direction producing maximum THG near interfaces. We then show that a finite-difference time-domain (FDTD) modeling strategy can accurately account for contrast observed in optically heterogeneous samples, whereas reference Fourier-based numerical approaches are accurate only in the absence of index mismatch. This work opens perspectives for interpreting THG microscopy images of tubular objects and other geometries.
Citation
Morizet , J , Olivier , N , Mahou , P , Boutillon , A , Stringari , C & Beaurepaire , E 2023 , ' Third harmonic imaging contrast from tubular structures in the presence of index discontinuity ' , Scientific Reports , vol. 13 , 7850 . https://doi.org/10.1038/s41598-023-34528-7
Publication
Scientific Reports
Status
Peer reviewed
DOI
https://doi.org/10.1038/s41598-023-34528-7
ISSN
2045-2322
Type
Journal article
Rights
Copyright © The Author(s) 2023. Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.
Description
Funding: Agence Nationale de la Recherche (ANR) (ANR-EQPX-0029, ANR-10-INBS-04, ANR-15-CE11-0012).
Collections
  • University of St Andrews Research
URI
http://hdl.handle.net/10023/27634

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