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dc.contributor.authorBuddawong, Tiranan
dc.contributor.authorAsuvapongpatana, Somluk
dc.contributor.authorSuwannasing, Chanyatip
dc.contributor.authorHabuddha, Valainipha
dc.contributor.authorSukonset, Chompoonut
dc.contributor.authorSombutkayasith, Chanyarak
dc.contributor.authorMcDougall, Carmel
dc.contributor.authorWeerachatyanukul, Wattana
dc.identifier.citationBuddawong , T , Asuvapongpatana , S , Suwannasing , C , Habuddha , V , Sukonset , C , Sombutkayasith , C , McDougall , C & Weerachatyanukul , W 2021 , ' Calcineurin subunit B is involved in shell regeneration in Haliotis diversicolor ' , PeerJ , vol. 9 , e10662 .
dc.identifier.otherPURE: 283589783
dc.identifier.otherPURE UUID: 7c54f5dc-3ffc-4a88-94ef-113bc50f664f
dc.identifier.otherPubMed: 33520456
dc.identifier.otherPubMedCentral: PMC7810044
dc.identifier.otherScopus: 85099361519
dc.descriptionFunding: This study was supported by the Thailand Research Fund (TRF) through a Royal Golden Jubilee Ph.D. Program (Grant No. PHD/0047/2554).en
dc.description.abstractAbalone shells are mainly composed of two major polymorphs of CaCO3 that are distributed in different layers of the shell. The process of shell biomineralization is controlled by genes and proteins expressed within the mantle epithelium. In this present paper, we conducted a shell regeneration experiment to study the role of HcCNA and HcCNB (individual subunits of calcineurin) in shell biomineralization in H. diversicolor. The results of qPCR showed that HcCNB is upregulated to a greater extent than HcCNA in the mantle after shell notching. In vivo study of the effects of rHcCNB injection showed a significantly higher percentage of regenerated shell length, but not area, in the injected group compared to the control group. In addition, SEM observation of the inner surface of the regenerated shells revealed three different zones including prismatic, nacreous, and a distinct transition zone. Changes in the crystal organization and ultrastructure are clearly evident in these three zones, particularly after 3 weeks of rHcCNB administration. We hypothesize that this is due to faster biomineralization rates in the rHcCNB treated group. Taken together, our results demonstrate that HcCNB participates in shell regeneration in H. diversicolor. As calcineurin subunits have also been implicated in shell formation in bivalves, these findings suggest that calcineurin subunits may play important roles in biomineralization in all conchiferans.
dc.rightsCopyright © 2021 Buddawong et al. Distributed under Creative Commons CC-BY 4.0.en
dc.subjectHaliotis diversicoloren
dc.subjectQH426 Geneticsen
dc.subjectSH Aquaculture. Fisheries. Anglingen
dc.titleCalcineurin subunit B is involved in shell regeneration in Haliotis diversicoloren
dc.typeJournal articleen
dc.description.versionPublisher PDFen
dc.contributor.institutionUniversity of St Andrews. School of Biologyen
dc.description.statusPeer revieweden

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