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dc.contributor.authorWojnowska, Marta
dc.contributor.authorFeng, Xuan
dc.contributor.authorChen, Yawen
dc.contributor.authorDeng, Hai
dc.contributor.authorO'Hagan, David
dc.identifier.citationWojnowska , M , Feng , X , Chen , Y , Deng , H & O'Hagan , D 2023 , ' Identification of genes essential for fluorination and sulfamylation within the nucleocidin gene clusters of Streptomyces calvus and Streptomyces virens ' , ChemBioChem , vol. 24 , no. 5 , e202200684 .
dc.identifier.otherPURE: 282683466
dc.identifier.otherPURE UUID: 782fab2f-0873-4552-869a-29866dcee13b
dc.identifier.otherRIS: urn:C399E57B0D21585DADAB0C4FFA6461E5
dc.identifier.otherORCID: /0000-0002-0510-5552/work/127573511
dc.identifier.otherWOS: 000921398700001
dc.identifier.otherScopus: 85146824149
dc.descriptionFunding: Authors thank the EPSRC, Leverhulme Trust and the EU Horizon 2020 Sinfonia consortia for financial support. The Chinese Scholarship Council is acknowledged for studentship support (Y.C.).en
dc.description.abstractThe gene cluster in Streptomyces calvus associated with the biosynthesis of the fluoro- and sulfamyl-metabolite nucleocidin was interrogated by systematic gene knockouts. Out of the 26 gene deletions most did not affect fluorometabolite production, nine abolished sulfamylation but not fluorination, and three precluded fluorination, having no effect on sulfamylation. In addition to nucI, nucG, nucJ, nucK, nucL, nucN, nucO, nucQ and nucP we identified two genes (nucW, nucA), belonging to a phosphoadenosine phosphosulfate (PAPS) gene cluster, as required for sulfamyl assembly. Three genes (orf(-3), orf2 and orf3) were found to be essential for fluorination, although the activities of their protein products are unknown. These genes as well as nucK, nucN, nucO and nucPNP, whose knockouts produced results differing from those described in a recent report, were also deleted in Streptomyces virens - with confirmatory outcomes. This genetic profile should inform biochemistry aimed at uncovering the enzymology behind nucleocidin biosynthesis.
dc.rightsCopyright © 2022 The Authors. ChemBioChem published by Wiley-VCH GmbH. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.en
dc.subjectGene knockoutsen
dc.subjectFluorometabolite biosynthesisen
dc.subjectNatural productsen
dc.subjectQD Chemistryen
dc.titleIdentification of genes essential for fluorination and sulfamylation within the nucleocidin gene clusters of Streptomyces calvus and Streptomyces virensen
dc.typeJournal articleen
dc.contributor.sponsorEuropean Commissionen
dc.contributor.sponsorThe Leverhulme Trusten
dc.description.versionPublisher PDFen
dc.contributor.institutionUniversity of St Andrews. School of Chemistryen
dc.contributor.institutionUniversity of St Andrews. Institute of Behavioural and Neural Sciencesen
dc.contributor.institutionUniversity of St Andrews. EaSTCHEMen
dc.contributor.institutionUniversity of St Andrews. Biomedical Sciences Research Complexen
dc.description.statusPeer revieweden

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