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dc.contributor.authorLo, Yi Ting
dc.contributor.authorRyan, Martin Denis
dc.contributor.authorLuke, Garry Alec
dc.contributor.authorChang, Wan Chen
dc.contributor.authorWu, Hsing Chieh
dc.identifier.citationLo , Y T , Ryan , M D , Luke , G A , Chang , W C & Wu , H C 2023 , ' Immunogenicity of a secreted, C‑terminally truncated, form of bovine viral diarrhea virus E2 glycoprotein as a potential candidate in subunit vaccine development. ' , Scientific Reports , vol. 13 , 296 .
dc.identifier.otherPURE: 282860397
dc.identifier.otherPURE UUID: 8ae43770-0ba2-433f-85e7-826ee470b8da
dc.identifier.otherORCID: /0000-0002-0012-0614/work/126554268
dc.identifier.otherScopus: 85145841846
dc.descriptionFunding: This work was supported by the Taiwan Ministry of Science and Technology [MOST-106-2911-I-020-501; MOST-107-2313-B-020-011-MY3] and the UK Biotechnology and Biological Sciences Research Council [BB/P025080/1].en
dc.description.abstractBoth current live, attenuated, and killed virus vaccines for bovine viral diarrhea virus (BVDV) have their limitations. Here, we report the development of a BVDV subunit vaccine by (i) the expression of a secreted form of a recombinant E2 glycoprotein using BHK21 cells and (ii) determination of the immune responses in mice. The E2 glycoprotein was modified by deletion of the C-terminal transmembrane anchor domain and fusion to a V5 epitope tag. This allowed detection using anti-V5 monoclonal antibodies together with simple purification of the expressed, secreted, form of E2 from the cell media. Furthermore, we genetically fused green fluorescent protein (GFP) linked to E2 via a Thosea asigna virus 2A (T2A) ribosome skipping sequence thereby creating a self-processing polyprotein [GFP-T2A-BVDV-E2trunk-V5], producing discrete [GFP-T2A] and [E2trunk-V5] translation products: GFP fluorescence acts, therefore, as a surrogate marker of E2 expression, BALB/c mice were inoculated with [E2trunk-V5] purified from cell media and both humoral and cellular immune responses were observed. Our antigen expression system provides, therefore, both (i) a simple antigen purification protocol together with (ii) a feasible strategy for further, large-scale, production of vaccines.
dc.relation.ispartofScientific Reportsen
dc.rightsCopyright © 2023 The Author(s). This article is licensed under a Creative Commons Attribution 4.0. International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit
dc.subjectSubunit vaccinesen
dc.subjectQH301 Biologyen
dc.subjectQR355 Virologyen
dc.subjectRM Therapeutics. Pharmacologyen
dc.subjectSDG 3 - Good Health and Well-beingen
dc.titleImmunogenicity of a secreted, C‑terminally truncated, form of bovine viral diarrhea virus E2 glycoprotein as a potential candidate in subunit vaccine development.en
dc.typeJournal articleen
dc.description.versionPublisher PDFen
dc.contributor.institutionUniversity of St Andrews. School of Biologyen
dc.description.statusPeer revieweden

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