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dc.contributor.authorLo, Yi Ting
dc.contributor.authorRyan, Martin Denis
dc.contributor.authorLuke, Garry Alec
dc.contributor.authorChang, Wan Chen
dc.contributor.authorWu, Hsing Chieh
dc.date.accessioned2023-01-09T17:30:05Z
dc.date.available2023-01-09T17:30:05Z
dc.date.issued2023-01-06
dc.identifier282860397
dc.identifier8ae43770-0ba2-433f-85e7-826ee470b8da
dc.identifier85145841846
dc.identifier.citationLo , Y T , Ryan , M D , Luke , G A , Chang , W C & Wu , H C 2023 , ' Immunogenicity of a secreted, C‑terminally truncated, form of bovine viral diarrhea virus E2 glycoprotein as a potential candidate in subunit vaccine development. ' , Scientific Reports , vol. 13 , 296 . https://doi.org/10.1038/s41598-022-26766-yen
dc.identifier.issn2045-2322
dc.identifier.otherORCID: /0000-0002-0012-0614/work/126554268
dc.identifier.urihttps://hdl.handle.net/10023/26726
dc.descriptionFunding: This work was supported by the Taiwan Ministry of Science and Technology [MOST-106-2911-I-020-501; MOST-107-2313-B-020-011-MY3] and the UK Biotechnology and Biological Sciences Research Council [BB/P025080/1].en
dc.description.abstractBoth current live, attenuated, and killed virus vaccines for bovine viral diarrhea virus (BVDV) have their limitations. Here, we report the development of a BVDV subunit vaccine by (i) the expression of a secreted form of a recombinant E2 glycoprotein using BHK21 cells and (ii) determination of the immune responses in mice. The E2 glycoprotein was modified by deletion of the C-terminal transmembrane anchor domain and fusion to a V5 epitope tag. This allowed detection using anti-V5 monoclonal antibodies together with simple purification of the expressed, secreted, form of E2 from the cell media. Furthermore, we genetically fused green fluorescent protein (GFP) linked to E2 via a Thosea asigna virus 2A (T2A) ribosome skipping sequence thereby creating a self-processing polyprotein [GFP-T2A-BVDV-E2trunk-V5], producing discrete [GFP-T2A] and [E2trunk-V5] translation products: GFP fluorescence acts, therefore, as a surrogate marker of E2 expression, BALB/c mice were inoculated with [E2trunk-V5] purified from cell media and both humoral and cellular immune responses were observed. Our antigen expression system provides, therefore, both (i) a simple antigen purification protocol together with (ii) a feasible strategy for further, large-scale, production of vaccines.
dc.format.extent11
dc.format.extent2144171
dc.language.isoeng
dc.relation.ispartofScientific Reportsen
dc.subjectSubunit vaccinesen
dc.subjectBVDVen
dc.subjectE2en
dc.subjectGlycoproteinen
dc.subject2Aen
dc.subjectQH301 Biologyen
dc.subjectQR355 Virologyen
dc.subjectRM Therapeutics. Pharmacologyen
dc.subjectDASen
dc.subjectSDG 3 - Good Health and Well-beingen
dc.subjectMCCen
dc.subject.lccQH301en
dc.subject.lccQR355en
dc.subject.lccRMen
dc.titleImmunogenicity of a secreted, C‑terminally truncated, form of bovine viral diarrhea virus E2 glycoprotein as a potential candidate in subunit vaccine development.en
dc.typeJournal articleen
dc.contributor.sponsorBBSRCen
dc.contributor.institutionUniversity of St Andrews. School of Biologyen
dc.identifier.doi10.1038/s41598-022-26766-y
dc.description.statusPeer revieweden
dc.identifier.grantnumberBB/P025080/1en


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