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dc.contributor.authorZhou, Liang
dc.contributor.authorLei, Qi
dc.contributor.authorGuo, Jimin
dc.contributor.authorGao, Yuanyuan
dc.contributor.authorShi, Jianjun
dc.contributor.authorYu, Hong
dc.contributor.authorYin, Wenxiang
dc.contributor.authorCao, Jiangfan
dc.contributor.authorXiao, Botao
dc.contributor.authorAndreo, Jacopo
dc.contributor.authorEttlinger, Romy
dc.contributor.authorJeffrey Brinker, C
dc.contributor.authorWuttke, Stefan
dc.contributor.authorZhu, Wei
dc.date.accessioned2022-11-04T11:30:14Z
dc.date.available2022-11-04T11:30:14Z
dc.date.issued2022-10-21
dc.identifier282006267
dc.identifieref90b747-f844-4901-b663-c7f2e39e35a8
dc.identifier36270991
dc.identifier85140297991
dc.identifier000871124000043
dc.identifier.citationZhou , L , Lei , Q , Guo , J , Gao , Y , Shi , J , Yu , H , Yin , W , Cao , J , Xiao , B , Andreo , J , Ettlinger , R , Jeffrey Brinker , C , Wuttke , S & Zhu , W 2022 , ' Long-term whole blood DNA preservation by cost-efficient cryosilicification ' , Nature Communications , vol. 13 , 6265 . https://doi.org/10.1038/s41467-022-33759-yen
dc.identifier.issn2041-1723
dc.identifier.otherJisc: 702135
dc.identifier.otherpii: 10.1038/s41467-022-33759-y
dc.identifier.otherpmc: PMC9587218
dc.identifier.urihttps://hdl.handle.net/10023/26303
dc.descriptionFunding: This work was supported by the National Natural Science Foundation of China (21972047 to W.Z., 52003086 to Q.L.), Guangdong Provincial Pearl River Talents Program (2019QN01Y314 to Q.L.), the Program for Guangdong Introducing Innovative and Entrepreneurial Teams (2019ZT08Y318 to W.Z.), Natural Science Foundation of Guangdong Province, China (2021A1515010724 to Q.L.), China Postdoctoral Science Foundation (2020M672625, 2021T140213 to Q.L.), Science and Technology Project of Guangzhou, China (202102020352 to W.Z., 202102020259 to Q.L.), the Fundamental Research Funds for the Central Universities of China. The authors thank the support from the Guangzhou Women and Children’s Medical Center and Laboratory Animal Research Center of the South China University of Technology. S.W. acknowledges funding from the Basque Government Industry Department under the ELKARTEK and HAZITEK programs.en
dc.description.abstractDeoxyribonucleic acid (DNA) is the blueprint of life, and cost-effective methods for its long-term storage could have many potential benefits to society. Here we present the method of in situ cryosilicification of whole blood cells, which allows long-term preservation of DNA. Importantly, our straightforward approach is inexpensive, reliable, and yields cryosilicified samples that fulfill the essential criteria for safe, long-term DNA preservation, namely robustness against external stressors, such as radical oxygen species or ultraviolet radiation, and long-term stability in humid conditions at elevated temperatures. Our approach could enable the room temperature storage of genomic information in book-size format for more than one thousand years (thermally equivalent), costing only 0.5 $/person. Additionally, our demonstration of 3D-printed DNA banking artefacts, could potentially allow 'artificial fossilization'.
dc.format.extent14
dc.format.extent2777945
dc.language.isoeng
dc.relation.ispartofNature Communicationsen
dc.subjectPreservation, Biological - methodsen
dc.subjectBlood Preservation - methodsen
dc.subjectOxygenen
dc.subjectUltraviolet Raysen
dc.subjectDNA - geneticsen
dc.subjectHumansen
dc.subjectQH426 Geneticsen
dc.subjectDASen
dc.subjectMCCen
dc.subject.lccQH426en
dc.titleLong-term whole blood DNA preservation by cost-efficient cryosilicificationen
dc.typeJournal articleen
dc.contributor.institutionUniversity of St Andrews. School of Chemistryen
dc.identifier.doi10.1038/s41467-022-33759-y
dc.description.statusPeer revieweden


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