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dc.contributor.authorChen, Yawen
dc.contributor.authorZhang, Qingzhi
dc.contributor.authorFeng, Xuan
dc.contributor.authorWojnowska, Marta
dc.contributor.authorO'Hagan, David
dc.date.accessioned2022-11-03T00:40:58Z
dc.date.available2022-11-03T00:40:58Z
dc.date.issued2021-11-03
dc.identifier.citationChen , Y , Zhang , Q , Feng , X , Wojnowska , M & O'Hagan , D 2021 , ' Streptomyces aureorectus DSM 41692 and Streptomyces virens DSM 41465 are producers of the antibiotic nucleocidin and 4'-fluoroadenosine is identified as a co-product ' , Organic & Biomolecular Chemistry , vol. Advance Article . https://doi.org/10.1039/d1ob01898aen
dc.identifier.issn1477-0520
dc.identifier.otherPURE: 276736953
dc.identifier.otherPURE UUID: d05f19e5-01d6-46f7-a6dd-4ba14ac33ba9
dc.identifier.otherPubMed: 34779476
dc.identifier.otherORCID: /0000-0002-0510-5552/work/103510899
dc.identifier.otherScopus: 85120685092
dc.identifier.otherWOS: 000718417700001
dc.identifier.urihttps://hdl.handle.net/10023/26294
dc.descriptionAuthors thank the Leverhulme Trust, EPSRC and the Sinfonia consortium of the EU for grants as well as the Chinese Scholarship Council for a Studentship (YC), all of which helped to support this work.en
dc.description.abstractGenome homology and the presence of a putative biosynthetic gene cluster identified Streptomyces aureorectus DSM 41692 and Streptomyces virens DSM 41465 as candidate producers of the antibiotic nucleocidin 1. Indeed when these bacterial strains were cultured in a medium supplemented with fluoride (4 mM) they each produced nucleocidin 1 and the previously identified 4'-fluoro-3'-O-β-glucosylated adenosine 2 and its sulfamylated derivative 3. In both of these cases 4'-fluoroadenosine 9 is also identified as a natural product although it has never been observed during fermentations of Streptomyces calvus, the original source of nucleocidin 1. The identity of 4'-fluoroadenosine 9 was confirmed by a total synthesis as well as by its in vitro enzymatic conversion to metabolite 2 using the glucosyl transferase enzyme, NucGT.
dc.format.extent4
dc.language.isoeng
dc.relation.ispartofOrganic & Biomolecular Chemistryen
dc.rightsCopyright © 2021 The Author(s). This work has been made available online in accordance with publisher policies or with permission. Permission for further reuse of this content should be sought from the publisher or the rights holder. This is the author created accepted manuscript following peer review and may differ slightly from the final published version. The final published version of this work is available at https://doi.org/10.1039/D1OB01898Aen
dc.subjectQD Chemistryen
dc.subjectNDASen
dc.subjectACen
dc.subject.lccQDen
dc.titleStreptomyces aureorectus DSM 41692 and Streptomyces virens DSM 41465 are producers of the antibiotic nucleocidin and 4'-fluoroadenosine is identified as a co-producten
dc.typeJournal articleen
dc.contributor.sponsorEuropean Commissionen
dc.description.versionPostprinten
dc.description.versionPostprinten
dc.contributor.institutionUniversity of St Andrews. School of Chemistryen
dc.contributor.institutionUniversity of St Andrews. Biomedical Sciences Research Complexen
dc.contributor.institutionUniversity of St Andrews. EaSTCHEMen
dc.identifier.doihttps://doi.org/10.1039/d1ob01898a
dc.description.statusPeer revieweden
dc.date.embargoedUntil2022-11-03
dc.identifier.grantnumber814418en


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