Show simple item record

Files in this item

Thumbnail

Item metadata

dc.contributor.authorYagoub, Suliman H
dc.contributor.authorLim, Megan
dc.contributor.authorTan, Tiffany C Y
dc.contributor.authorChow, Darren J X
dc.contributor.authorDholakia, Kishan
dc.contributor.authorGibson, Brant C
dc.contributor.authorThompson, Jeremy G
dc.contributor.authorDunning, Kylie R
dc.date.accessioned2022-08-26T16:30:14Z
dc.date.available2022-08-26T16:30:14Z
dc.date.issued2022-08-11
dc.identifier281024877
dc.identifier285a03d4-df78-4405-9b80-86a839b9ce66
dc.identifier35951146
dc.identifier85135812280
dc.identifier000839330500001
dc.identifier.citationYagoub , S H , Lim , M , Tan , T C Y , Chow , D J X , Dholakia , K , Gibson , B C , Thompson , J G & Dunning , K R 2022 , ' Vitrification within a nanoliter volume : oocyte and embryo cryopreservation within a 3D photopolymerized device ' , Journal of Assisted Reproduction and Genetics , vol. First Online . https://doi.org/10.1007/s10815-022-02589-8en
dc.identifier.issn1058-0468
dc.identifier.otherJisc: 556293
dc.identifier.otherpii: 10.1007/s10815-022-02589-8
dc.identifier.urihttps://hdl.handle.net/10023/25900
dc.descriptionOpen Access funding enabled and organized by CAUL and its Member Institutions. KRD is supported by a Mid-Career Fellowship from the Hospital Research Foundation (C-MCF-58–2019). KD acknowledges funding from the UK Engineering and Physical Sciences Research Council (grants EP/P030017/1). This study was funded by the Australian Research Council (ARC) Centre of Excellence for Nanoscale BioPhotonics (CE140100003).en
dc.description.abstractPurpose Vitrification permits long-term banking of oocytes and embryos. It is a technically challenging procedure requiring direct handling and movement of cells between potentially cytotoxic cryoprotectant solutions. Variation in adherence to timing, and ability to trace cells during the procedure, affects survival post-warming. We hypothesized that minimizing direct handling will simplify the procedure and improve traceability. To address this, we present a novel photopolymerized device that houses the sample during vitrification. Methods The fabricated device consisted of two components: the Pod and Garage. Single mouse oocytes or embryos were housed in a Pod, with multiple Pods docked into a Garage. The suitability of the device for cryogenic application was assessed by repeated vitrification and warming cycles. Oocytes or early blastocyst-stage embryos were vitrified either using standard practice or within Pods and a Garage and compared to non-vitrified control groups. Post-warming, we assessed survival rate, oocyte developmental potential (fertilization and subsequent development) and metabolism (autofluorescence). Results Vitrification within the device occurred within ~ 3 nL of cryoprotectant: this volume being ~ 1000-fold lower than standard vitrification. Compared to standard practice, vitrification and warming within our device showed no differences in viability, developmental competency, or metabolism for oocytes and embryos. The device housed the sample during processing, which improved traceability and minimized handling. Interestingly, vitrification-warming itself, altered oocyte and embryo metabolism. Conclusion The Pod and Garage system minimized the volume of cryoprotectant at vitrification—by ~ 1000-fold—improved traceability and reduced direct handling of the sample. This is a major step in simplifying the procedure.
dc.format.extent18
dc.format.extent14811031
dc.language.isoeng
dc.relation.ispartofJournal of Assisted Reproduction and Geneticsen
dc.subjectOocyteen
dc.subjectVitrificationen
dc.subjectPhotopolymerizationen
dc.subjectIVFen
dc.subject3D fabricationen
dc.subjectEmbryoen
dc.subjectMetabolismen
dc.subjectQH301 Biologyen
dc.subjectQH426 Geneticsen
dc.subjectNDASen
dc.subject.lccQH301en
dc.subject.lccQH426en
dc.titleVitrification within a nanoliter volume : oocyte and embryo cryopreservation within a 3D photopolymerized deviceen
dc.typeJournal articleen
dc.contributor.sponsorEPSRCen
dc.contributor.institutionUniversity of St Andrews. School of Physics and Astronomyen
dc.contributor.institutionUniversity of St Andrews. Sir James Mackenzie Institute for Early Diagnosisen
dc.contributor.institutionUniversity of St Andrews. Centre for Biophotonicsen
dc.contributor.institutionUniversity of St Andrews. Institute of Behavioural and Neural Sciencesen
dc.contributor.institutionUniversity of St Andrews. Biomedical Sciences Research Complexen
dc.identifier.doihttps://doi.org/10.1007/s10815-022-02589-8
dc.description.statusPeer revieweden
dc.identifier.grantnumberEP/P030017/1en


This item appears in the following Collection(s)

Show simple item record