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dc.contributor.authorVersace, Elisabetta
dc.contributor.authorSgadò, Paola
dc.contributor.authorGeorge, Julia
dc.contributor.authorLoveland, Jasmine L.
dc.contributor.authorWard, Joseph
dc.contributor.authorThorpe, Peter
dc.contributor.authorJensen, Lars Juhl
dc.contributor.authorSpencer, Karen A.
dc.contributor.authorParacchini, Silvia
dc.contributor.authorVallortigara, Giorgio
dc.date.accessioned2022-07-28T09:30:03Z
dc.date.available2022-07-28T09:30:03Z
dc.date.issued2022-07-15
dc.identifier280633000
dc.identifier1e228595-3ced-4233-ad65-2058011dbd45
dc.identifier85134202231
dc.identifier000826110000005
dc.identifier.citationVersace , E , Sgadò , P , George , J , Loveland , J L , Ward , J , Thorpe , P , Jensen , L J , Spencer , K A , Paracchini , S & Vallortigara , G 2022 , ' Light-induced asymmetries in embryonic retinal gene expression are mediated by the vascular system and extracellular matrix ' , Scientific Reports , vol. 12 , 12086 . https://doi.org/10.1038/s41598-022-14963-8en
dc.identifier.issn2045-2322
dc.identifier.otherRIS: urn:964FD17650B8D85546B1122E40C24434
dc.identifier.otherORCID: /0000-0002-2851-9379/work/116598034
dc.identifier.otherORCID: /0000-0001-9934-8602/work/116598355
dc.identifier.urihttps://hdl.handle.net/10023/25733
dc.descriptionFunding: This project was supported by BBSRC Grant BB/S003223/1 (EV, JG), Royal Society Grant UF150663 (SP), Novo Nordisk Foundation Grant NNF14CC0001 (LJJ), ERC Horizon 2020 Grant Agreement 833504 SPANUMBRA, PRIN 2017 and ERC-SH4-A (2017PSRHPZ) to GV.en
dc.description.abstractLeft–right asymmetries in the nervous system (lateralisation) influence a broad range of behaviours, from social responses to navigation and language. The role and pathways of endogenous and environmental mechanisms in the ontogeny of lateralisation remains to be established. The domestic chick is a model of both endogenous and experience-induced lateralisation driven by light exposure. Following the endogenous rightward rotation of the embryo, the asymmetrical position in the egg results in a greater exposure of the right eye to environmental light. To identify the genetic pathways activated by asymmetric light stimulation, and their time course, we exposed embryos to different light regimes: darkness, 6 h of light and 24 h of light. We used RNA-seq to compare gene expression in the right and left retinas and telencephalon. We detected differential gene expression in right vs left retina after 6 h of light exposure. This difference was absent in the darkness condition and had already disappeared by 24 h of light exposure, suggesting that light-induced activation is a self-terminating phenomenon. This transient effect of light exposure was associated with a downregulation of the sensitive-period mediator gene DIO2 (iodothyronine deiodinase 2) in the right retina. No differences between genes expressed in the right vs. left telencephalon were detected. Gene networks associated with lateralisation were connected to vascularisation, cell motility, and the extracellular matrix. Interestingly, we know that the extracellular matrix—including the differentially expressed PDGFRB gene—is involved in morphogenesis, sensitive periods, and in the endogenous chiral mechanism of primary cilia, that drives lateralisation. Our data show a similarity between endogenous and experience-driven lateralisation, identifying functional gene networks that affect lateralisation in a specific time window.
dc.format.extent12
dc.format.extent3083882
dc.language.isoeng
dc.relation.ispartofScientific Reportsen
dc.subjectQH426 Geneticsen
dc.subjectDASen
dc.subjectMCCen
dc.subject.lccQH426en
dc.titleLight-induced asymmetries in embryonic retinal gene expression are mediated by the vascular system and extracellular matrixen
dc.typeJournal articleen
dc.contributor.institutionUniversity of St Andrews. School of Medicineen
dc.contributor.institutionUniversity of St Andrews. St Andrews Bioinformatics Uniten
dc.contributor.institutionUniversity of St Andrews. Institute of Behavioural and Neural Sciencesen
dc.contributor.institutionUniversity of St Andrews. Coastal Resources Management Groupen
dc.contributor.institutionUniversity of St Andrews. School of Psychology and Neuroscienceen
dc.contributor.institutionUniversity of St Andrews. Centre for Biophotonicsen
dc.contributor.institutionUniversity of St Andrews. Cellular Medicine Divisionen
dc.contributor.institutionUniversity of St Andrews. Biomedical Sciences Research Complexen
dc.identifier.doi10.1038/s41598-022-14963-8
dc.description.statusPeer revieweden


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