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dc.contributor.authorCampugan, Carl A
dc.contributor.authorLim, Megan
dc.contributor.authorChow, Darren J X
dc.contributor.authorTan, Tiffany C Y
dc.contributor.authorLi, Tong
dc.contributor.authorSaini, Avishkar A
dc.contributor.authorOrth, Antony
dc.contributor.authorReineck, Philipp
dc.contributor.authorSchartner, Erik P
dc.contributor.authorThompson, Jeremy G
dc.contributor.authorDholakia, Kishan
dc.contributor.authorDunning, Kylie R
dc.date.accessioned2022-06-30T12:30:14Z
dc.date.available2022-06-30T12:30:14Z
dc.date.issued2022-06-23
dc.identifier.citationCampugan , C A , Lim , M , Chow , D J X , Tan , T C Y , Li , T , Saini , A A , Orth , A , Reineck , P , Schartner , E P , Thompson , J G , Dholakia , K & Dunning , K R 2022 , ' The effect of discrete wavelengths of visible light on the developing murine embryo ' , Journal of Assisted Reproduction and Genetics , vol. First Online . https://doi.org/10.1007/s10815-022-02555-4en
dc.identifier.issn1058-0468
dc.identifier.otherPURE: 280299967
dc.identifier.otherPURE UUID: 772c59c3-2b35-4e78-ac87-f6510ef3f238
dc.identifier.otherPubMed: 35737174
dc.identifier.otherScopus: 85132564391
dc.identifier.urihttp://hdl.handle.net/10023/25589
dc.descriptionOpen Access funding enabled and organized by CAUL and its Member Institutions KRD is supported by a Mid-Career Fellowship from the Hospital Research Foundation (C-MCF-58–2019). KD is supported by funding from the UK Engineering and Physical Sciences Research Council (EP/P030017/1) and the Australian Research Council (FL210100099). CC acknowledges the support of a PhD scholarship jointly from the University of Adelaide and University of Nottingham. This study was funded by the Australian Research Council Centre of Excellence for Nanoscale BioPhotonics (CE140100003). PR acknowledges funding through the RMIT Vice-Chancellor’s Research Fellowship and ARC DECRA Fellowship scheme (DE200100279).en
dc.description.abstractPurpose A current focus of the IVF field is non-invasive imaging of the embryo to quantify developmental potential. Such approaches use varying wavelengths to gain maximum biological information. The impact of irradiating the developing embryo with discrete wavelengths of light is not fully understood. Here, we assess the impact of a range of wavelengths on the developing embryo. Methods Murine preimplantation embryos were exposed daily to wavelengths within the blue, green, yellow, and red spectral bands and compared to an unexposed control group. Development to blastocyst, DNA damage, and cell number/allocation to blastocyst cell lineages were assessed. For the longer wavelengths (yellow and red), pregnancy/fetal outcomes and the abundance of intracellular lipid were investigated. Results Significantly fewer embryos developed to the blastocyst stage when exposed to the yellow wavelength. Elevated DNA damage was observed within embryos exposed to blue, green, or red wavelengths. There was no effect on blastocyst cell number/lineage allocation for all wavelengths except red, where there was a significant decrease in total cell number. Pregnancy rate was significantly reduced when embryos were irradiated with the red wavelength. Weight at weaning was significantly higher when embryos were exposed to yellow or red wavelengths. Lipid abundance was significantly elevated following exposure to the yellow wavelength. Conclusion Our results demonstrate that the impact of light is wavelength-specific, with longer wavelengths also impacting the embryo. We also show that effects are energy-dependent. This data shows that damage is multifaceted and developmental rate alone may not fully reflect the impact of light exposure.
dc.format.extent13
dc.language.isoeng
dc.relation.ispartofJournal of Assisted Reproduction and Geneticsen
dc.rightsCopyright © The Author(s) 2022. This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.en
dc.subjectPhotodamageen
dc.subjectPhototoxicityen
dc.subjectMicroscopyen
dc.subjectPreimplantation embryoen
dc.subjectBlastocysten
dc.subjectQH301 Biologyen
dc.subjectRG Gynecology and obstetricsen
dc.subjectNDASen
dc.subject.lccQH301en
dc.subject.lccRGen
dc.titleThe effect of discrete wavelengths of visible light on the developing murine embryoen
dc.typeJournal articleen
dc.contributor.sponsorEPSRCen
dc.description.versionPublisher PDFen
dc.contributor.institutionUniversity of St Andrews. School of Physics and Astronomyen
dc.contributor.institutionUniversity of St Andrews. Sir James Mackenzie Institute for Early Diagnosisen
dc.contributor.institutionUniversity of St Andrews. Centre for Biophotonicsen
dc.contributor.institutionUniversity of St Andrews. Institute of Behavioural and Neural Sciencesen
dc.contributor.institutionUniversity of St Andrews. Biomedical Sciences Research Complexen
dc.identifier.doihttps://doi.org/10.1007/s10815-022-02555-4
dc.description.statusPeer revieweden
dc.identifier.grantnumberEP/P030017/1en


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