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dc.contributor.authorvan Steenbrugge, Joris J. M.
dc.contributor.authorvan den Elsen, Sven
dc.contributor.authorHolterman, Martijn
dc.contributor.authorLozano-Torres, Jose L.
dc.contributor.authorPutker, Vera
dc.contributor.authorThorpe, Peter
dc.contributor.authorGoverse, Aska
dc.contributor.authorSterken, Mark G.
dc.contributor.authorSmant, Geert
dc.contributor.authorHelder, Johannes
dc.date.accessioned2022-06-01T16:30:20Z
dc.date.available2022-06-01T16:30:20Z
dc.date.issued2022-06-01
dc.identifier279613584
dc.identifier301ea22d-809d-4f6b-b9bc-dcd1620d1296
dc.identifier85131176039
dc.identifier000803868500001
dc.identifier.citationvan Steenbrugge , J J M , van den Elsen , S , Holterman , M , Lozano-Torres , J L , Putker , V , Thorpe , P , Goverse , A , Sterken , M G , Smant , G & Helder , J 2022 , ' Comparative genomics among cyst nematodes reveals distinct evolutionary histories among effector families and an irregular distribution of effector-associated promoter motifs ' , Molecular Ecology , vol. Early View , 16505 . https://doi.org/10.1111/mec.16505en
dc.identifier.issn0962-1083
dc.identifier.otherRIS: urn:D3539A323909BCA799BE3A5A2E34E287
dc.identifier.urihttps://hdl.handle.net/10023/25481
dc.descriptionJvS, MH and SvdE were supported by a grant from the Applied and Technical Science domain (TTW) of the Netherlands Organization for Scientific Research (NWO) under grant no. 14708. PT received support from the University of St Andrews Bioinformatics Unit (AMD3BIOINF), funded by Wellcome Trust ISSF award 105621/Z/14/Z. MS benefitted from funding by a VENI grant (17282) from the NWO domain Applied and Engineering Sciences.en
dc.description.abstractPotato cyst nematodes (PCNs), an umbrella term used for two species, Globodera pallida and G. rostochiensis, belong worldwide to the most harmful pathogens of potato. Pathotype-specific host plant resistances are an essential handle for PCN control. However, the poor delineation of G. pallida pathotypes hampers the efficient use of available host plant resistances. Long-read sequencing technology allowed us to generate a new reference genome of G. pallida population D383 and, as compared to the current reference, the new genome assembly is 42 times less fragmented. For comparison of diversification patterns of six effector families between G. pallida and G. rostochiensis, an additional reference genome was generated for an outgroup, the beet cyst nematode Heterodera schachtii (IRS population). Large evolutionary contrasts in effector family topologies were observed. While VAPs diversified before the split between the three cyst nematode species, the families GLAND5 and GLAND13 only expanded in PCN after their separation from the genus Heterodera. Although DNA motifs in the promoter regions thought to be involved in the orchestration of effector expression ('DOG boxes') were present in all three cyst nematode species, their presence is not a necessity for dorsal gland-produced effectors. Notably, DOG box dosage was only loosely correlated with expression level of individual effector variants. Comparison of the G. pallida genome with those of two other cyst nematodes underlined the fundamental differences in evolutionary history between effector families. Re-sequencing of PCN populations with deviant virulence characteristics will allow for the linking of these characteristics with the composition of the effector repertoire as well as for the mapping of PCN diversification patterns resulting from extreme anthropogenic range expansion.
dc.format.extent2662119
dc.language.isoeng
dc.relation.ispartofMolecular Ecologyen
dc.subjectCyst nematodesen
dc.subjectDOG boxen
dc.subjectGloboderaen
dc.subjectHeteroderaen
dc.subjectNematode effectorsen
dc.subjectQH426 Geneticsen
dc.subjectQR Microbiologyen
dc.subjectDASen
dc.subject.lccQH426en
dc.subject.lccQRen
dc.titleComparative genomics among cyst nematodes reveals distinct evolutionary histories among effector families and an irregular distribution of effector-associated promoter motifsen
dc.typeJournal articleen
dc.contributor.sponsorThe Wellcome Trusten
dc.contributor.institutionUniversity of St Andrews. School of Medicineen
dc.contributor.institutionUniversity of St Andrews. Institute of Behavioural and Neural Sciencesen
dc.contributor.institutionUniversity of St Andrews. St Andrews Bioinformatics Uniten
dc.identifier.doi10.1111/mec.16505
dc.description.statusPeer revieweden
dc.identifier.grantnumber105621/Z/14/Zen


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