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Hyperexcitability in young iPSC-derived C9ORF72 mutant motor neurons is associated with increased intracellular calcium release

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Burley_2022_SciRep_Hyperexcitability_young_iPSC_derived_C9ORF72_mutant_CC.pdf (2.038Mb)
Date
05/05/2022
Author
Burley, Sarah
Beccano-Kelly, Dayne A.
Talbot, Kevin
Cordero Llana, Oscar
Wade-Martins, Richard
Keywords
QH426 Genetics
QP Physiology
RC0321 Neuroscience. Biological psychiatry. Neuropsychiatry
3rd-DAS
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Abstract
A large hexanucleotide repeat expansion in the C9ORF72 gene is the most prevalent cause of amyotrophic lateral sclerosis (ALS). To better understand neuronal dysfunction during ALS progression, we studied motor neuron (MN) cultures derived from iPSC lines generated from C9ORF72 (C9) expansion carriers and unaffected controls. C9 and control MN cultures showed comparable mRNA levels for MN markers SMI-32, HB9 and ISL1 and similar MN yields (> 50% TUJ1/SMI-32 double-positive MNs). Using whole-cell patch clamp we showed that C9-MNs have normal membrane capacitance, resistance and resting potential. However, immature (day 40) C9-MNs exhibited a hyperexcitable phenotype concurrent with increased release of calcium (Ca2+) from internal stores, but with no changes to NaV and KV currents. Interestingly, this was a transient phenotype. By day 47, maturing C9-MNs demonstrated normal electrophysiological activity, displaying only subtle alterations on mitochondrial Ca2+ release. Together, these findings suggest the potential importance of a developmental component to C9ORF72-related ALS.
Citation
Burley , S , Beccano-Kelly , D A , Talbot , K , Cordero Llana , O & Wade-Martins , R 2022 , ' Hyperexcitability in young iPSC-derived C9ORF72 mutant motor neurons is associated with increased intracellular calcium release ' , Scientific Reports , vol. 12 , 7378 . https://doi.org/10.1038/s41598-022-09751-3
Publication
Scientific Reports
Status
Peer reviewed
DOI
https://doi.org/10.1038/s41598-022-09751-3
ISSN
2045-2322
Type
Journal article
Rights
Copyright © The Author(s) 2022. This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder.
Description
S.B. was supported by a Motor Neuron Disease Association studentship (WadeMartins/Oct13/867-792) and D.A.B was supported by the Monument Trust Discovery Award (J-1403) from Parkinson’s UK. The work was supported by the Medical Research Council Dementias Platform UK Stem Cell Network (Grant MR/M024962/1 to RW-M).
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  • University of St Andrews Research
URI
http://hdl.handle.net/10023/25327

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